Skip to main content
SpringerLink
Log in

Characterisation of 22 polymorphic microsatellite loci for the Broadbill Swordfish, Xiphias gladius

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The Broadbill Swordfish is harvested by fisheries throughout the world. In order to devise more effective management strategies, we need a clear understanding of the population structure of the species. From a library of 29 tetranucleotide repeats, 22 microsatellite markers were characterised for 94 swordfish samples captured from eastern and western Australia. The number of alleles ranged from 2 to 26 and observed heterozygosity from 0.066 to 0.923. We have identified 18 microsatellites that will be valuable in the examination of swordfish population structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Alvarado Bremer JR, Mejuto J, Greig TW, Ely B (1996) Global population structure of the swordfish (Xiphias gladius L.) as revealed by analysis of the mitochondrial DNA control region. J Exp Mar Biol Ecol 197(2):295–310

    Article  Google Scholar 

  • Alvarado Bremer JR, Mejuto J, Gómez-Márquez J, Boán F, Carpintero P, Rodríguez JM, Viñas J, Greig TW, Ely B (2005) Hierarchical analyses of genetic variation of samples from breeding and feeding grounds confirm the genetic partitioning of northwest Atlantic and South Atlantic populations of swordfish (Xiphias gladius L.). J Exp Mar Biol Ecol 327(2):167–182

    Article  CAS  Google Scholar 

  • Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(6):1004–1006

    CAS  PubMed  Google Scholar 

  • Chow S, Takeyama H (2000) Nuclear and mitochondrial DNA analyses reveal four genetically separated breeding units of the swordfish. J Fish Biol 56(5):1087–1098

    Article  CAS  Google Scholar 

  • Chow S, Okamoto H, Uozumi Y, Takeuchi Y, Takeyama H (1997) Genetic stock structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the mitochondrial DNA control region. Mar Biol 127(3):359–367

    Article  Google Scholar 

  • Greig TW, Alvarado Bremer JR, Ely B (2000) Nuclear markers provide additional evidence for population subdivision among Atlantic swordfish. Intern Comm Conserv Atl Tunas Collect Vol Sci Pap 51(5):1637–1640

    Google Scholar 

  • Jean C, Bourjea J, Jouen E, Taquet M (2006) Stock structure of the swordfish (Xiphias gladius) in the southwest Indian Ocean: a preliminary study. Bull Mar Sci 79(3):521–526

    Google Scholar 

  • Kasapidis P, Mejuto J, Tserpes G, Antoniou A, Garcia-Cortes B, Peristeraki P, Oikonomaki K, Kotoulas G, Magoulas A (2007) Genetic structure of the swordfish (Xiphias gladius) stocks in the Atlantic using microsatellite DNA analysis. Intern Comm Conserv Atl Tunas Collect Vol Sci Pap 61:89–98

    Google Scholar 

  • Kasapidis P, Magoulas A, Garcia-Cortes B, Mejuto J (2008) Stock structure of swordfish (Xiphias gladius) in the Pacific Ocean using microsatellite DNA markers. Biology Specialist Working Group Paper WCPFC-SC4-2008/BI WP-4 4th Regular Session of the Western and Central Pacific Fisheries Commission. 11–22 Aug 2008

  • Kasapidis P, Pakaki V, Kotoulas G, Magoulas A (2009) Isolation and characterization of 18 new polymorphic microsatellite loci for the swordfish, Xiphias gladius. Mol Ecol Resour 9(5):1383–1386

    Article  Google Scholar 

  • Kotoulas G, Mejuto J, Antoniou A, Kasapidis P, Tserpes G, Piccinetti C, Peristeraki P, Garcia-Cortes B, Oikonomaki K, de la Serna JM, Magoulas A (2007) Global genetic structure of swordfish (Xiphias gladius) as revealed by microsatellite DNA markers. Intern Comm Conserv Atl Tunas Collect Vol Sci Pap 61:79–88

    Google Scholar 

  • Muths D, Grewe P, Jean C, Bourjea J (2009) Genetic population structure of the swordfish (Xiphias gladius) in the southwest Indian Ocean: sex-biased differentiation, congruency between markers and its incidence in a way of stock assessment. Fish Res 97(3):263–269

    Article  Google Scholar 

  • Peakall R, Smouse P (2006) GenAlEx 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295

    Article  Google Scholar 

  • Reeb CA, Arcangeli L, Block BA (2003) Development of 11 microsatellite loci for population studies in the swordfish, Xiphias gladius (Teleostei: Scombridae). Mol Ecol Notes 3(1):147–149

    Article  CAS  Google Scholar 

  • Rosel PE, Block BA (1996) Mitochondrial control region variability and global population structure in the swordfish, Xiphias gladius. Mar Biol 125(1):11–22

    Article  CAS  Google Scholar 

  • Schable NA, Fischer RU, Glenn TC (2002) Tetranucleotide microsatellite DNA loci from the dollar sunfish (Lepomis marginatus). Mol Ecol Notes 2(4):509–511

    Article  CAS  Google Scholar 

  • Ward RD (2000) Genetics in fisheries management. Hydrobiologia 420(1):191–201

    Article  CAS  Google Scholar 

  • Ward RD, Reeb CA, Block BA (2001) Population structure of Australian swordfish, Xiphias gladius. Final Report to the Australian Fisheries Management Authority

Download references

Acknowledgments

We thank K. Evans, M. Lansdell, and C. Burns for sample collection. Funding was provided by CSIRO Wealth from Oceans National Research Flagship, CSIRO Marine and Atmospheric Research, by European Funds: European Fisheries Funds EU FEP 2007–2013 (project IOSSS-ESPADON) and the EU 7th Funding Program—Capacities, Research Potential (project RUNSeaSciences).

Author information

Authors and Affiliations

  1. Department of Genetics, University of Melbourne, Royal Parade, Parkville, VIC, 3010, Australia

    H. Bradman & B. Appleton

  2. CSIRO Wealth from Oceans National Research Flagship, CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart, TAS, 7001, Australia

    P. Grewe

  3. IFREMER Délégation de La Réunion, Rue Jean Bertho, BP60, 97822, Le Port, France

    J. Bourjea & D. Muths

Authors
  1. H. Bradman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Grewe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Bourjea
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Muths
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Appleton
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Bradman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bradman, H., Grewe, P., Bourjea, J. et al. Characterisation of 22 polymorphic microsatellite loci for the Broadbill Swordfish, Xiphias gladius . Conservation Genet Resour 3, 263–266 (2011). https://doi.org/10.1007/s12686-010-9337-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-010-9337-8

Keywords

Search

Navigation