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Isolation and characterization of microsatellite loci for the oval pigtoe mussel, Pleurobema pyriforme (Lea 1857)

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Abstract

We isolated nine microsatellite loci from Pleurobema pyriforme, a freshwater mussel species currently protected under the Endangered Species Act. Eight loci were polymorphic with 2–15 alleles and observed heterozygosities of 0.130–0.957. All loci conformed to Hardy–Weinberg expectations and all pairs of loci showed no significant genotypic disequilibrium. These are the first isolated microsatellite loci for this species and will serve in ongoing conservation efforts of P. pyriforme.

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Acknowledgments

We thank S. Abbott, B. Dudely, K. Herrington, S. Pursifull, and C. Stringfellow for collection of mussel samples and Genetic Identification Services for construction and sequencing of enriched microsatellite libraries. Use of trade names throughout the manuscript does not constitute endorsement by the United States government.

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Authors and Affiliations

  1. Warm Springs Fish Technology Center, Conservation Genetics Laboratory, US Fish and Wildlife Service, 5308 Spring Street, Warm Springs, GA, 31830, USA

    G. R. Moyer & A. S. Williams

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  1. G. R. Moyer
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  2. A. S. Williams
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Correspondence to G. R. Moyer.

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Moyer, G.R., Williams, A.S. Isolation and characterization of microsatellite loci for the oval pigtoe mussel, Pleurobema pyriforme (Lea 1857). Conservation Genet Resour 3, 255–257 (2011). https://doi.org/10.1007/s12686-010-9335-x

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  • DOI: https://doi.org/10.1007/s12686-010-9335-x

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