Abstract
Primers for 10 microsatellite loci were developed specifically to amplify low quantity and quality DNA in the endangered Indiana Bat (Myotis sodalis). In a screen of 20 individuals from a population in Missouri, the 10 loci were found to have levels of variability ranging from seven to 18 alleles. No loci were found to be linked, although two loci revealed significant departures from Hardy–Weinberg equilibrium. These microsatellite loci will be applicable for population genetic analyses and for use in mark-recapture studies that utilize DNA collected non-invasively from fecal pellets, which will ultimately aid in management efforts.
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Acknowledgments
This work was funded by the US Geological Survey in collaboration with the Fish and Wildlife Service. The use of any trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. We thank S. Amelon for providing DNA samples, and J. Whitaker and D. Sparks for providing tissue samples. Additionally, we thank J. St John for her technical help on this project and L. Ellison and A. Piaggio for their helpful comments on this manuscript.
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Oyler-McCance, S.J., Fike, J.A. Characterization of small microsatellite loci isolated in endangered Indiana bat (Myotis sodalis) for use in non-invasive sampling. Conservation Genet Resour 3, 243–245 (2011). https://doi.org/10.1007/s12686-010-9332-0
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DOI: https://doi.org/10.1007/s12686-010-9332-0