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Identification and characterization of nuclear microsatellite loci for multiple species of chorus frogs (Pseudacris) for population genetic analyses

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Abstract

Microsatellites were developed from an enriched genomic library of Pseudacris feriarum and P. nigrita. For 85 P. feriarum, six loci averaged 59 alleles, with loci ranging from 42–71 alleles. Observed heterozygosity averaged 0.72/locus (range: 0.42–0.84). In 142 P. maculata, seven additional loci (plus two loci above) averaged 8 alleles, with loci ranging from 3–16 alleles. Observed heterozygosity averaged 0.60/locus (range: 0.35–0.81).

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References

  • Lemmon AR, Lemmon EM (2008) A likelihood framework for estimating phylogeographic history on a continuous landscape. Syst Biol 57:544–561

    Article  PubMed  Google Scholar 

  • Lemmon EM (2009) Diversification of conspecific signals in sympatry: geographic overlap drives multidimensional reproductive character displacement in frogs. Evolution 63:1155–1170

    Article  PubMed  Google Scholar 

  • Lemmon EM, Lemmon AR (2010) Reinforcement in chorus frogs: lifetime fitness estimates including intrinsic natural selection and sexual selection against hybrids. Evolution 64:1748–1761

    Article  PubMed  Google Scholar 

  • Lemmon EM, Lemmon AR, Collins JT, Lee-Yaw JA, Cannatella DC (2007a) Phylogeny-based delimitation of species boundaries and contact zones in the trilling chorus frogs (Pseudacris). Mol Phylogenet Evol 44:1068–1082

    Article  PubMed  Google Scholar 

  • Lemmon EM, Lemmon AR, Cannatella DC (2007b) Geological and climatic forces driving speciation in the continentally distributed trilling chorus frogs (Pseudacris). Evolution 61:2086–2103

    Article  PubMed  Google Scholar 

  • Matthews JH, Boles S, Parmesan C, Juenger TE (2007) Isolation and characterization of nuclear microsatellite loci for the common green darner dragonfly Anax junius (Odonata: Aeshnidae) to constrain patterns of phenotypic and spatial diversity. Mol Ecol Notes 7:845–847

    Article  CAS  Google Scholar 

  • Moriarty EC, Cannatella DC (2004) Phylogenetic relationships of the North American chorus frogs (Pseudacris: Hylidae). Mol Phylogenet Evol 30:409–420

    Article  CAS  PubMed  Google Scholar 

  • Murphy MA, Evans JS, Storfer A (2010) Quantifying Bufo boreas connectivity in Yellowstone National Park with landscape genetics. Ecology 91:252–261

    Article  PubMed  Google Scholar 

  • Rousset F (2008) ‘GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux’. Mol Ecol Resources 8:103–106

    Article  Google Scholar 

  • Van Oosterhout C, Weetman D, Hutchinson WF (2006) Estimation and adjustment of microsatellite null alleles in nonequilibrium populations. Mol Ecol Notes 6:255–256

    Article  Google Scholar 

Download references

Acknowledgments

We thank Sandra Boles, Heather Hurston, Tierney Wayne, and Alan Lemmon. This research was supported by an NSF GRF and DDIG (0309309) to EML; NSF DDIG (0608458), EPA-STAR fellowship (FP-916695), and James King Fellowship to MAM; NSF IRCEB Grant 0078150 to David Cannatella; and NSF DEB 0546316 to TEJ.

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Authors and Affiliations

  1. Section of Integrative Biology, University of Texas at Austin, 1 University Station C0930, Austin, TX, 78712, USA

    Emily Moriarty Lemmon & Thomas E. Juenger

  2. Department of Renewable Resources, Ag. 2010, University of Wyoming, 1000 E University Ave., Laramie, WY, 82071, USA

    Melanie Murphy

  3. Department of Biological Science, Florida State University, 319 Stadium Drive, Tallahassee, FL, 32306, USA

    Emily Moriarty Lemmon

Authors
  1. Emily Moriarty Lemmon
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  2. Melanie Murphy
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  3. Thomas E. Juenger
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Corresponding author

Correspondence to Emily Moriarty Lemmon.

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Lemmon, E.M., Murphy, M. & Juenger, T.E. Identification and characterization of nuclear microsatellite loci for multiple species of chorus frogs (Pseudacris) for population genetic analyses. Conservation Genet Resour 3, 233–237 (2011). https://doi.org/10.1007/s12686-010-9330-2

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  • DOI: https://doi.org/10.1007/s12686-010-9330-2

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