Isolation and characterization of eight microsatellite loci in Chaetodon ornatissimus and cross-amplification in a sympatric sister species, Chaetodon meyeri
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Eight polymorphic microsatellite loci were developed for sister species, Ornate butterflyfish (Chaetodon ornatissimus) and Scrawled butterflyfish (Chaetodon meyeri). All loci were scored from samples collected at geographically distinct locations (C. ornatissimus, N = 37, Republic of Palau; C. meyeri, N = 30, Republic of Kiribati). There were 14.00 or 13.50 mean alleles per locus (A) for C. ornatissimus and C. meyeri, respectively, with observed heterozygosity (H O) ranging from 0.61 to 0.97 and 0.60 to 1.00. Most loci conformed to Hardy–Weinberg expectations, were in linkage equilibrium, and lacked confounding null alleles. We expect these markers to be useful for resolving population structure and patterns of hybridization.
KeywordsBiogeography Coral reef fishes Hybrid zone Indo-Pacific Phylogeography Sympatry
This research was supported by the National Science Foundation grants OCE-0453167 and OCE-0929031 to BWB, NOAA National Marine Sanctuaries Program MOA No. 2005-008/66882 to R.J. Toonen, and by a Natural Sciences and Engineering Research Council of Canada (NSERC) postgraduate fellowship to JDD. Microsatellite development was performed in the Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation. For specimen collections we thank Kim Andersen, Paul H. Barber, J. Howard Choat, G. Concepcion, Matthew T. Craig, Toby S. Daly-Engel, Joshua A. Drew, John L. Earle, Jeff Eble, Kevin Flanagan, Michelle R. Gaither, Brian D. Greene, Matthew Iacchei, Stephen A. Karl, Randall K. Kosaki, Carl G. Meyer, Yannis P. Papastamatiou, David Pence, Richard Pyle, Joshua Reece, Luiz A. Rocha, D. Ross Robertson, Jennifer K. Schultz, Derek Skillings, Derek Smith, Zoltan Szabo, Kim Tenggardjaja, Bill Walsh, Ivor Willliams, Jill P. Zamzow, and the crew of the R.V. Hi’ialakai. We also thank Robert Toonen, Serges Planes, Ben Victor, Hawaii Department of Land and Natural Resources, the Coral Reef Research Foundation and the Papahānaumokuākea Marine National Monument, U.S. Fish and Wildlife Service, members of the ToBo lab for logistic support; and the Center for Genomics, Proteomics, and Bioinformatics at the University of Hawaii (Manoa Campus) for their assistance with genotyping. This is contribution no. 1410 from the Hawai’i Institute of Marine Biology and no. 8024 from the School of Ocean and Earth Science and Technology.
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