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Conservation Genetics Resources

, Volume 3, Issue 2, pp 213–216 | Cite as

Isolation and characterization of eight microsatellite loci in Chaetodon ornatissimus and cross-amplification in a sympatric sister species, Chaetodon meyeri

  • Joseph D. DiBattistaEmail author
  • Kevin A. Feldheim
Technical Note

Abstract

Eight polymorphic microsatellite loci were developed for sister species, Ornate butterflyfish (Chaetodon ornatissimus) and Scrawled butterflyfish (Chaetodon meyeri). All loci were scored from samples collected at geographically distinct locations (C. ornatissimus, N = 37, Republic of Palau; C. meyeri, N = 30, Republic of Kiribati). There were 14.00 or 13.50 mean alleles per locus (A) for C. ornatissimus and C. meyeri, respectively, with observed heterozygosity (H O) ranging from 0.61 to 0.97 and 0.60 to 1.00. Most loci conformed to Hardy–Weinberg expectations, were in linkage equilibrium, and lacked confounding null alleles. We expect these markers to be useful for resolving population structure and patterns of hybridization.

Keywords

Biogeography Coral reef fishes Hybrid zone Indo-Pacific Phylogeography Sympatry 

Notes

Acknowledgments

This research was supported by the National Science Foundation grants OCE-0453167 and OCE-0929031 to BWB, NOAA National Marine Sanctuaries Program MOA No. 2005-008/66882 to R.J. Toonen, and by a Natural Sciences and Engineering Research Council of Canada (NSERC) postgraduate fellowship to JDD. Microsatellite development was performed in the Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation. For specimen collections we thank Kim Andersen, Paul H. Barber, J. Howard Choat, G. Concepcion, Matthew T. Craig, Toby S. Daly-Engel, Joshua A. Drew, John L. Earle, Jeff Eble, Kevin Flanagan, Michelle R. Gaither, Brian D. Greene, Matthew Iacchei, Stephen A. Karl, Randall K. Kosaki, Carl G. Meyer, Yannis P. Papastamatiou, David Pence, Richard Pyle, Joshua Reece, Luiz A. Rocha, D. Ross Robertson, Jennifer K. Schultz, Derek Skillings, Derek Smith, Zoltan Szabo, Kim Tenggardjaja, Bill Walsh, Ivor Willliams, Jill P. Zamzow, and the crew of the R.V. Hi’ialakai. We also thank Robert Toonen, Serges Planes, Ben Victor, Hawaii Department of Land and Natural Resources, the Coral Reef Research Foundation and the Papahānaumokuākea Marine National Monument, U.S. Fish and Wildlife Service, members of the ToBo lab for logistic support; and the Center for Genomics, Proteomics, and Bioinformatics at the University of Hawaii (Manoa Campus) for their assistance with genotyping. This is contribution no. 1410 from the Hawai’i Institute of Marine Biology and no. 8024 from the School of Ocean and Earth Science and Technology.

References

  1. Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele sizing methods. Bio Techniques 31:24–28Google Scholar
  2. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222CrossRefPubMedGoogle Scholar
  3. Hobbs JPA, Frisch AJ, Allen GR, van Herwerden L (2009) Marine hybrid hotspot at Indo-Pacific biogeographic border. Biol Lett 5:258–261PubMedGoogle Scholar
  4. Hsu K, Chen J, Shao K (2007) Molecular phylogeny of Chaetodon (Teleostei: Chaetodontidae) in the Indo-West Pacific: evolution in geminate species pairs and species groups. Raffles Bull Zool 14:77–86Google Scholar
  5. McMillan WO, Weigt LA, Palumbi SR (1999) Color pattern evolution, assortative mating, and genetic differentiation in brightly colored butterflyfishes (Chaetodontidae). Evolution 53:247–260CrossRefGoogle Scholar
  6. Meeker ND, Hutchinson SA, Ho L, Trede NK (2007) Method for isolation of PCR-ready genomic DNA from zebrafish tissues. Bio Techniques 43:610–614Google Scholar
  7. Pyle RL, Randall JE (1994) A review of hybridization in marine angelfishes (Perciformes: Pomacanthidae). Env Biol Fishes 41:127–145Google Scholar
  8. Raymond M, Rousset F (1995) GENEPOP v. 1.2: population genetics software for exact tests and ecumenicism. J Heredity 83:248–249Google Scholar
  9. Rozen S, Skaletsky H (2000) Primer 3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386PubMedGoogle Scholar
  10. Sokal RR, Rohlf FJ (1994) Biometry. W H Freeman & Company, New YorkGoogle Scholar
  11. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  12. Yaakub SM, Bellwood DR, van Herwerden L, Walsh FM (2006) Hybridization in coral reef fishes: introgression and bi-directional gene exchange in Thalassoma (family Labridae). Mol Phylogenet Evol 40:84–100CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Hawaii Institute of Marine BiologyKane’oheUSA
  2. 2.Field Museum, Pritzker Laboratory for Molecular Systematics and EvolutionChicagoUSA

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