Abstract
Cyclura carinata, a critically endangered Caribbean rock iguana, now occupies less than 5% of its historic range. Remaining populations are genetically structured, but available tools are insufficient to identify taxonomic units. Ten polymorphic microsatellites isolated from C. carinata, and 19 loci developed for congeners are identified. All 29 loci were screened for variation in two populations with 20 or more individuals from each of two island populations, as well as 24 individuals that are representative of the species range.
This is a preview of subscription content, log in via an institution to check access.
References
An J, Sommer JA, Shore GD, Williamson JE, Brenneman RA, Louis EE (2004) Characterization of 20 microsatellite marker loci in the West Indian rock iguana (Cyclura nubila). Conserv Genet 5:121–125
Bryan JJ, Gerber GP, Welch ME, Stephen CL (2007) Re-evaluating the taxonomic status of the Booby Cay Iguana, Cyclura carinata bartschi. Copeia 2007:734–739
Don R, Cox P, Wainwright B, Baker K, Mattick J (1991) ‘Touchdown’ PCR to circumvent spurious priming during amplification. Nucleic Acids Res 19:4008
Gerber GP (2004) Cyclura carinata. In: IUCN red list of threatened species. IUCN—The World Conservation Union, Gland, Switzerland
Gerber GP, Iverson JB (2000) Turks Island iguana, Cyclura carinata carinata. In: Alberts A (ed) West Indian iguana: status survey and conservation action plan. IUCN, Gland, Switzerland, pp 15–18
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
Iverson JB (1979) Behavior and ecology of the rock iguana Cyclura carinata Bull Fla State Mus Biol Sci 24:175–358
Lau J, Alberts AC, Chemnick LG, Gerber GP, Jones KC, Mitchell AA, Ryder OA (2009) Isolation and characterization of 23 polymorphic microsatellite loci for a West Indian iguana (Cyclura pinguis) from the British Virgin Islands. Mol Ecol Resour 9:1412–1414
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Rosas KG, Pérez-Buitrago N, Acevedo JP, Martínez, Funk SM (2008) Development and characterization of 11 microsatellite loci for the Mona Island iguana (Cyclura cornuta stejnegeri). Mol Ecol Resour 8:825–827
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Welch ME (1997) The population genetic structure of Cyclura carinata carinata (Sauria: Iguanidae). M.S. Thesis, University of Tennessee
Welch ME, Gerber GP, Davis SK (2004) Genetic structure of the Turks and Caicos Rock Iguana and its implications for species conservation. In: Alberts AC, Carter RL, Hayes WH, Martins EP (eds) Iguanas, biology and conservation. University of California Press, Berkeley, pp 58–70
Acknowledgments
Samples were collected by G.P.G. and M.E.W. while supported by the Turks and Caicos National Trust with funds from the United Kingdom Foreign and Commonwealth Office. Molecular work was conducted in the laboratories of M.E.W. and L.E.W. with funding from the Department of Biological Sciences, the College of Arts and Sciences and the Office of Research and Economic Development at Mississippi State University. The Office of Research and Economic Development at Mississippi State University also supported this project via a Research Initiation Program grant to M.E.W. The Shackouls Honors College provided summer support to G.J.L. and A.H.G. Additional support was provided by an EPSCoR Undergraduate Research Scholarship to A.H.G, and a Northeast Mississippi Daily Journal grant to G.J.L.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Welch, M.E., Long, G.J., Berk, J.W. et al. Twenty-nine polymorphic microsatellite loci in Cyclura carinata, the Turks and Caicos Iguana, a critically endangered island endemic. Conservation Genet Resour 3, 209–212 (2011). https://doi.org/10.1007/s12686-010-9324-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9324-0