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Conservation Genetics Resources

, Volume 3, Issue 2, pp 205–208 | Cite as

Development of microsatellite loci for Syzygium paniculatum (Myrtaceae), a rare polyembryonic rainforest tree

  • Katie A. G. ThurlbyEmail author
  • Carolyn Connelly
  • Peter G. Wilson
  • Maurizio Rossetto
Technical Note

Abstract

Fourteen novel microsatellite loci were developed for the rare polyembryonic and potentially polyploid rainforest tree Syzygium paniculatum Gaertn. Markers were characterized using 40 individuals from populations along the NSW coast. The mean number of alleles amplified per locus was three (range 1–7, H E = 0.54 and H′ = 0.85). Low allelic variation may be explained by the polyembryonic nature of S. paniculatum seeds. These markers provide the opportunity to use nuclear microsatellite markers to study the population genetic structure and breeding system in S. paniculatum and related species.

Keywords

Syzygium Microsatellite loci Polyembryony Apomixis Polyploidy Clonal 

Notes

Acknowledgments

We would like to thank the Australian Flora Foundation for funding, Hannah McPherson and William Sherwin for technical input and The Ramaciotti Centre for Gene Function Analysis (University of New South Wales, Australia) for performing sequencing.

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Copyright information

© Her Majesty the Queen in Rights of Australia as represented by the State of NSW and the Department of Environment, Climate Change and Water NSW 2010

Authors and Affiliations

  • Katie A. G. Thurlby
    • 1
    • 2
    • 3
    Email author
  • Carolyn Connelly
    • 1
  • Peter G. Wilson
    • 1
  • Maurizio Rossetto
    • 1
  1. 1.National Herbarium of New South Wales, Botanic Gardens TrustSydneyAustralia
  2. 2.School of Biological, Earth, and Environmental SciencesUniversity of New South WalesSydneyAustralia
  3. 3.School of Biotechnology and Biomolecular SciencesUniversity of New South WalesSydneyAustralia

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