Abstract
We isolated new 10 polymorphic microsatellites from the tropical sea cucumber Stichopus chloronotus. These loci provide one class of variable genetic marker as the number of alleles ranged from 2 to 6 and the observed and expected heterozygosity ranged from 0.083 to 1.0 and from 0.081 to 0.724, respectively. We consider that these loci are potentially useful for revealing clones (resulting from asexual repro) and then participate in detailing the genetic structure and gene flow among S. chloronotus populations.
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Acknowledgments
This work was conducted in the framework of the SEA-WP Connectivity Project (project leader: Kazuo Nadaoka, Tokyo Institute of Technology, Japan), supported by the Global Environment Research Fund (D-0802) of the Ministry of the Environment, Japan and Grant-in-Aid for Scientific Research (A) (No. 21254002) and Grant-in-Aid-for Research Fellows (No. 2002461) of JSPS (The Japan Society for the Promotion of Science). We thank the team members from Nadaoka Laboratory (Tokyo Institute of Technology) and National Research Institute of Fisheries and Environment of Inland Sea (Fisheries Research Agency of Japan) who actively participate in the sampling and laboratory work, in particular Tsutomu Takino and Ritsuko Kubota.
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Taquet, C., Nagai, S., Yasuda, N. et al. First report of the development of microsatellite markers for a tropical sea cucumber (Stichopus chloronotus). Conservation Genet Resour 3, 201–203 (2011). https://doi.org/10.1007/s12686-010-9322-2
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DOI: https://doi.org/10.1007/s12686-010-9322-2