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Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot

Conservation Genetics Resources volume 3, pages 167–171 (2011)Cite this article

Abstract

Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2–20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.

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Acknowledgments

We thank Marika Karjalainen and Kirsi Kähkönen for assistance in laboratory, Jacquelin DeFaveri for comments, Kerala forest department for sampling permissions and all the people who helped in obtaining the samples. The samples were collected under licence from National Biodiversity Authority, India (licence #: NBA/TECH Appl/9/85/34/08/08-09/682). Our research was funded by Academy of Finland (grant #: 125092) and Indian Department of Biotechnology (BT/IN/Finnish/11/SG/2008).

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Authors and Affiliations

  1. Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, PO Box 65, 00014, Helsinki, Finland

    Abhilash Nair, Meng-Hua Li & Juha Merilä

  2. Chemical Biology, Rajiv Gandhi Centre for Biotechnology, PO Thycaud, Poojappura, Thiruvananthapuram, Kerala, 695 014, India

    K. Santhosh Kumar, Sanil George & Sujith V. Gopalan

  3. Division of Genetics and Physiology, Department of Biology, University of Turku, Vesilinnantie 5, 20014, Turku, Finland

    Erica H. Leder

Authors
  1. Abhilash Nair
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  2. K. Santhosh Kumar
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  3. Sanil George
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  4. Sujith V. Gopalan
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  5. Meng-Hua Li
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  6. Erica H. Leder
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  7. Juha Merilä
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Correspondence to Abhilash Nair.

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Nair, A., Kumar, K.S., George, S. et al. Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot. Conservation Genet Resour 3, 167–171 (2011). https://doi.org/10.1007/s12686-010-9315-1

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  • DOI: https://doi.org/10.1007/s12686-010-9315-1

Keywords

  • Amphibia
  • Endemic
  • Indirana beddomii
  • Microsatellite
  • Western Ghats