Abstract
Scatophagus argus is a recreational and commercial fish in East Asia. There is no genetic information about wild populations available so far. A first set of 15 microsatellite loci were developed from the species and characterized. The number of alleles per locus varied from 3 to 12 with the average of 6.6. Levels of observed heterozygosity (HO) per locus ranged from 0.421 to 0.876, and the expected heterozygosity (HE) per locus was from 0.497 to 0.931. No significant linkage disequilibrium was detected between loci, and no departure from Hardy–Weinberg equilibrium was observed except for the locus SA108. These polymorphic microsatellite markers would aid in the conservation and management in Scatophagus argus.
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References
Allen GR (1984) Scatophagidae. In: Fischer W, Bianchi G (eds) FAO species identification sheets for fishery purposes, Western Indian Ocean (Fishing Area 51). FAO, Rome
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechniques 24:796–802
Kharin VE, Milovankin PG (2008) A new finding of rare species Scatophagus argus (Scatophagidae) in Russian waters. J Ichthyol 48:822–824. doi:10.1134/S0032945208090130
Kozfkay CC, Campbell MR, Heindel JA (2008) A genetic evaluation of relatedness for broodstock management of captive, endangered Snake River sockeye salmon, Oncorhynchus nerka. Conserv Genet 9:1421–1430. doi:10.1007/s10592-007-9466-0
Lan GB, Yan B, Liao SN, Luo Y, Xie RZ (2005) Biology of spotted scat Scatophagus argus: a review. Fish Sci 24:39–41 (In Chinese)
Nakabo T (ed) (2002) Fishes of Japan with pectorial keys and species. Tokai University Press, Tokyo 1749 p
Palumbi SR (2003) Population genetics, demographic connectivity and the design of marine protected areas. Ecol Appl 13:146–158
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x
Saenz-Agudelo P, Jones GP, Thorrold SR, Planes S (2009) Estimating connectivity in marine populations: an empirical evaluation of assignment tests and parentage analysis under different gene flow scenarios. Mol Ecol 18:1765–1776. doi:10.1111/j.1365-294X.2009.04109.x
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x
Acknowledgments
This work was supported by Chinese National Fund 40776089, “Shu Guang” and “Dongfang” projects from Shanghai Municipal Education Commission and Shanghai Education Development Foundation.
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Liu, H., Zhang, J., Cai, Z. et al. Novel polymorphic microsatellite loci for the spotted scat Scatophagus argus . Conservation Genet Resour 2 (Suppl 1), 149–151 (2010). https://doi.org/10.1007/s12686-010-9279-1
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DOI: https://doi.org/10.1007/s12686-010-9279-1