Microsatellite markers isolated from the Mexican banded spring snail Mexipyrgus churinceanus


The first twelve novel unlinked polymorphic microsatellite DNA loci were isolated from the Mexican banded spring snail (Mexipyrgus churinceanus). Genetic variability of each locus was assessed using 25 individuals from a single population from Cuatro Ciénegas, Mexico. Mean observed heterozygosity varied from 0.14 to 0.92. The relatively high levels of detected polymorphism indicate that these markers will be useful for future population genetic studies of this Cuatro Ciénegas endemic species.

This is a preview of subscription content, log in to check access.


  1. Brookfield JFY (1996) A simple new method for estimating null allele frequency from heterozygote deficiency. Mol Ecol 5:453–455

    CAS  PubMed  Google Scholar 

  2. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed  Google Scholar 

  3. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    CAS  Article  Google Scholar 

  4. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222

    CAS  Article  PubMed  Google Scholar 

  5. Johnson SG (2005) Age, phylogeography and population structure of the microendemic banded spring snail, Mexipyrgus churinceanus. Mol Ecol 14:2299–2311

    CAS  Article  PubMed  Google Scholar 

  6. Rousset F (2008) GENEPOP ‘007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  Google Scholar 

  7. Wagner AP, Creel S, Kalinowski ST (2006) Estimating relatedness and relationships using microsatellite loci with null alleles. Heredity 97:336–345

    CAS  Article  PubMed  Google Scholar 

  8. Wigginton JE, Cutler DJ, Abecasis GR (2005) A note on exact tests of Hardy-Weinberg equilibrium. Am J Hum Genet 76:887–893

    CAS  Article  PubMed  Google Scholar 

  9. Wolaver BD, Sharp JM Jr, Rodriguez JM, Ibarra-Flores JC (2008) Delineation of regional arid karstic aquifers: an integrative data approach. Ground Water 46:396–413

    Google Scholar 

Download references


Our work was partially supported by the Undergraduate Mentoring in Environmental Biology Program at the University of New Orleans funded by the National Science Foundation. We thank the University of New Orleans, the Savannah River Ecology Laboratory, and the University of Georgia for support. The study was conducted with permission from the Mexican government (Permiso de Pesca de Fomento 230401-613-03 and DAN 02939).

Author information



Corresponding author

Correspondence to Steven G. Johnson.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Coghill, L.M., Chaves-Campos, J., Espinoza, F.E. et al. Microsatellite markers isolated from the Mexican banded spring snail Mexipyrgus churinceanus . Conservation Genet Resour 3, 29–31 (2011). https://doi.org/10.1007/s12686-010-9273-7

Download citation


  • Cuatro Ciénegas
  • Mexipyrgus churinceanus
  • Microsatellites
  • PCR
  • Primers