Conservation Genetics Resources

, Volume 3, Issue 1, pp 25–27 | Cite as

Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents

  • Thomas F. Schultz
  • Pen-Yuan Hsing
  • Allison Eng
  • Kevin A. Zelnio
  • Andrew David Thaler
  • Jens Carlsson
  • Cindy Lee Van Dover
Technical Note

Abstract

Species in the genus Bathymodiolus are mytilid mussels found at deep-sea hydrothermal vents and cold seeps. Next-generation sequencing techniques were employed to identify eighteen unlinked polymorphic microsatellite loci for Bathymodiolus manusensis from Manus Basin in the western Pacific. Allele frequencies for eight loci conform to Hardy–Weinberg expectations and observed heterozygosities ranged from 0.04 to 0.90 (mean HO = 0.45, SD = 0.25). Ten of eighteen loci cross-amplified in Bathymodiolus heckerae from Atlantic seeps. Microsatellites developed for Bathymodiolus manusensis are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.

Keywords

Mytilid mussel Back-arc basin Chemoautotrophic Bathymodiolus Hydrothermal vent Microsatellite 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Thomas F. Schultz
    • 1
  • Pen-Yuan Hsing
    • 1
  • Allison Eng
    • 1
  • Kevin A. Zelnio
    • 1
  • Andrew David Thaler
    • 1
  • Jens Carlsson
    • 1
    • 2
  • Cindy Lee Van Dover
    • 1
  1. 1.Marine Conservation Molecular FacilityDuke University Marine Laboratory, Nicholas School of the EnvironmentBeaufortUSA
  2. 2.Department of Zoology, Ecology and Plant ScienceUniversity College CorkCorkIreland

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