Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents
Species in the genus Bathymodiolus are mytilid mussels found at deep-sea hydrothermal vents and cold seeps. Next-generation sequencing techniques were employed to identify eighteen unlinked polymorphic microsatellite loci for Bathymodiolus manusensis from Manus Basin in the western Pacific. Allele frequencies for eight loci conform to Hardy–Weinberg expectations and observed heterozygosities ranged from 0.04 to 0.90 (mean H O = 0.45, SD = 0.25). Ten of eighteen loci cross-amplified in Bathymodiolus heckerae from Atlantic seeps. Microsatellites developed for Bathymodiolus manusensis are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.
KeywordsMytilid mussel Back-arc basin Chemoautotrophic Bathymodiolus Hydrothermal vent Microsatellite
This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples of Bathymodiolus heckerae were collected with support of the NOAA Ocean Exploration Program through an award to CLVD. Research by AE and P-Y H was supported in part by the Rachel Carson Scholars Program at the Duke University Marine Laboratory. We are grateful to Lisa Bukovnik in the IGSP at Duke University for performing the GS-FLX Titanium sequencing. Samples used in this study were collected on behalf of the people of Papua New Guinea.
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