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Isolation and characterization of 20 polymorphic microsatellite markers in Macrobrachium nipponense

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Abstract

We developed 20 polymorphic microsatellite loci by 5′-anchored PCR and constructing a microsatellite-enriched genomic library. The number of alleles per locus ranged from two to twelve, and observed and expected heterozygosities from 0.2357 to 1.0000 and from 0.4156 to 0.9169, respectively. Seven loci deviated from the HWE in the sampled population, null alleles were found in four loci, and gamete linkage disequilibrium between two loci was significant. These microsatellite markers will be useful for the study of population structure and genetic diversity of M. nipponense.

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Acknowledgments

This work was supported by the National Program for High Technology Research and Development in China (2008AA101008), Shandong Province Breeding Project for Agricultural Organisms (2007LZ013), and Shandong Province Natural Science Foundation (Y2008D32).

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Authors and Affiliations

  1. College of Animal Science and Technology, Shandong Agricultural University, Daizong Street 61, 271018, Taian, China

    Yan Zhao, Hui Wang, Xiangshan Ji, Yongqing Zeng, Pingping Yang & Lei Ding

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  1. Yan Zhao
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  2. Hui Wang
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  3. Xiangshan Ji
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  4. Yongqing Zeng
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  5. Pingping Yang
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  6. Lei Ding
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Corresponding author

Correspondence to Yongqing Zeng.

Additional information

Yan Zhao and Hui Wang are contributed equally.

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Zhao, Y., Wang, H., Ji, X. et al. Isolation and characterization of 20 polymorphic microsatellite markers in Macrobrachium nipponense . Conservation Genet Resour 2 (Suppl 1), 137–139 (2010). https://doi.org/10.1007/s12686-010-9263-9

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  • DOI: https://doi.org/10.1007/s12686-010-9263-9

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