Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents

Abstract

Species in the genus Chorocaris are caridean shrimp found at deep-sea hydrothermal vents at mid-ocean spreading centers. Nine unlinked polymorphic microsatellite loci were developed for this Chorocaris sp. 2 from the Manus back-arc basin, southwest Pacific. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.17 to 0.74 (mean HO = 0.45, SD = 0.21). Eight out of nine loci cross-amplified in one or two additional species of alvinocaridid shrimp that co-occur with Chorocaris sp. 2 at western Pacific vent habitats. The efficacy of our markers to detect genetic population structure is demonstrated using 362 individuals from 3 sites in Manus Basin. Microsatellites developed for Chorocaris sp. 2 are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.

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Acknowledgments

This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples used in this study were collected on behalf of the people of Papua New Guinea.

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Correspondence to K. A. Zelnio.

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Zelnio, K.A., Thaler, A.D., Jones, R.E. et al. Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents. Conservation Genet Resour 2, 223–226 (2010). https://doi.org/10.1007/s12686-010-9243-0

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Keywords

  • Alvinocarididae
  • Back-arc Basin
  • Chemosynthetic-based ecosystem
  • Chorocaris
  • Hydrothermal vent
  • Shrimp
  • Microsatellite