Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents
- 113 Downloads
Species in the genus Chorocaris are caridean shrimp found at deep-sea hydrothermal vents at mid-ocean spreading centers. Nine unlinked polymorphic microsatellite loci were developed for this Chorocaris sp. 2 from the Manus back-arc basin, southwest Pacific. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.17 to 0.74 (mean HO = 0.45, SD = 0.21). Eight out of nine loci cross-amplified in one or two additional species of alvinocaridid shrimp that co-occur with Chorocaris sp. 2 at western Pacific vent habitats. The efficacy of our markers to detect genetic population structure is demonstrated using 362 individuals from 3 sites in Manus Basin. Microsatellites developed for Chorocaris sp. 2 are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.
KeywordsAlvinocarididae Back-arc Basin Chemosynthetic-based ecosystem Chorocaris Hydrothermal vent Shrimp Microsatellite
This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples used in this study were collected on behalf of the people of Papua New Guinea.
- Carney SL, Formica MI, Divatia H, Nelson K, Fisher CR, Schaeffer SW (2006) Population structure of the mussel “Bathymodiolus” childressi from Gulf of Mexico hydrocarbon seeps. Deep Sea Res I 53:1061–1072Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Thaler AD, Zelnio KA, Jones RE, Carlsson J, Van Dover CL, Schultz TF (2010) Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Cons Gen Resour. DOI: 10.1007/s12686-010-9174-9
- Thurston MI, Field D (2005) Msatfinder: detection and characterisation of microsatellites. Distributed by the authors at http://www.genomics.ceh.ac.uk/msatfinder/. CEH Oxford, Mansfield Road, Oxford OX1 3SR
- Valière N (2002) GIMLET: a computer program for analysing genetic individual identification data. Mol Ecol Notes 2:377–379Google Scholar
- Woods JG, Paetkau D, Lewis D, McLellan BN, Proctor M, Strobeck C (1999) Genetic tagging of free-ranging black and brown bears. Wildl Soc Bull 27:616–627Google Scholar