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Conservation Genetics Resources

, Volume 2, Supplement 1, pp 73–75 | Cite as

Isolation of polymorphic microsatellite loci from an endangered freshwater species Chinese sucker, Myxocyprinus asiaticus

  • Nan Chen
  • Huan Ling WangEmail author
  • Wei Min Wang
  • Vibeke Simonsen
Technical Note

Abstract

Chinese sucker (Myxocyprinus asiaticus) is an endangered freshwater fish species endemic to the Yangtze River in China. In this work, we described the isolation and characterization of 14 new polymorphic microsatellite loci from (CA) n and (GA) n microsatellite enriched libraries of the M. asiaticus. The number of alleles per locus ranged from 6 to 14 with the observed (H O) and expected (H E) heterozygosities ranging from 0.120 to 1.000 and from 0.767 to 0.952, respectively. Five loci displayed significant departures from Hardy–Weinberg expectations. These polymorphic microsatellite loci will be useful molecular tools for understanding population genetic structure and conservation of M. asiaticus.

Keywords

Chinese sucker Myxocyprinus asiaticus Microsatellites Endangered species Conservation genetics 

Notes

Acknowledgments

This research was supported by International Fundation of Science (A/4525-1) and Natural Science Foundation of Hubei province of China (No. 2008CBB102).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Nan Chen
    • 1
  • Huan Ling Wang
    • 1
    Email author
  • Wei Min Wang
    • 1
  • Vibeke Simonsen
    • 2
  1. 1.Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of FisheryHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.National Environment Research InstituteSilkeborgDenmark

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