Isolation of polymorphic microsatellite loci from an endangered freshwater species Chinese sucker, Myxocyprinus asiaticus
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Chinese sucker (Myxocyprinus asiaticus) is an endangered freshwater fish species endemic to the Yangtze River in China. In this work, we described the isolation and characterization of 14 new polymorphic microsatellite loci from (CA) n and (GA) n microsatellite enriched libraries of the M. asiaticus. The number of alleles per locus ranged from 6 to 14 with the observed (H O) and expected (H E) heterozygosities ranging from 0.120 to 1.000 and from 0.767 to 0.952, respectively. Five loci displayed significant departures from Hardy–Weinberg expectations. These polymorphic microsatellite loci will be useful molecular tools for understanding population genetic structure and conservation of M. asiaticus.
KeywordsChinese sucker Myxocyprinus asiaticus Microsatellites Endangered species Conservation genetics
This research was supported by International Fundation of Science (A/4525-1) and Natural Science Foundation of Hubei province of China (No. 2008CBB102).