Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents

Abstract

Ifremeria nautilei is deep-sea provannid gastropod endemic to hydrothermal vents at southwest Pacific back-arc spreading centers. Twelve, selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.719 to 0.906 (mean H O  = 0.547, SD = 0.206). Three of the 12 loci cross-amplified in two species of Alviniconcha (Provannidae) that co-occur with I. nautilei at Pacific vent habitats. Microsatellites developed for I. nautilei are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.

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Acknowledgments

This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples used in this study were collected on behalf of the people of Papua New Guinea.

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Correspondence to Andrew David Thaler.

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Thaler, A.D., Zelnio, K., Jones, R. et al. Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conservation Genet Resour 2, 101–103 (2010). https://doi.org/10.1007/s12686-010-9174-9

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Keywords

  • Back-arc basin
  • Chemoautotrophic
  • Hydrothermal vent
  • Ifremeria nautilei
  • Microsatellite
  • Provannid gastropod