Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents
- 120 Downloads
Ifremeria nautilei is deep-sea provannid gastropod endemic to hydrothermal vents at southwest Pacific back-arc spreading centers. Twelve, selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.719 to 0.906 (mean HO = 0.547, SD = 0.206). Three of the 12 loci cross-amplified in two species of Alviniconcha (Provannidae) that co-occur with I. nautilei at Pacific vent habitats. Microsatellites developed for I. nautilei are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.
KeywordsBack-arc basin Chemoautotrophic Hydrothermal vent Ifremeria nautilei Microsatellite Provannid gastropod
- Bouchet P, Warén A (1991) Ifremeria nautilei, a new gastropod from hydrothermal vents, probably associated with symbiotic bacteria. C R Acad Sci III 312(10):495–501Google Scholar
- Desbruyères D, Hashimoto J, Fabri MC (2006) Composition and biogeography of hydrothermal vent communities in western Pacific back-arc basins. Geophys Monogr 166:215–234Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Thurston MI, Field D (2005) Msatfinder: detection and characterisation of microsatellites. Distributed by the authors at http://www.genomics.ceh.ac.uk/msatfinder/. CEH Oxford, Mansfield Road, Oxford OX1 3SR