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Conservation Genetics Resources

, Volume 2, Supplement 1, pp 59–61 | Cite as

The isolation and characterization of 10 dinucleotide microsatellite markers from enriched Channa argus genomic library

  • Yasmeen Gul
  • Weimin WangEmail author
  • Huanling Wang
Technical Note

Abstract

Eleven microsatellite markers were isolated and characterized from enriched genomic libraries of Channa argus. The loci were scored in 40 specimens from one natural population. We explored 6–17 alleles per locus with observed and expected heterozygosities ranging from 0.8000 to 1.0000 and 0.7269 to 0.9551, respectively, containing 0.830 average polymorphic information content. Significant deviations from Hardy–Weinberg equilibrium were detected only at one loci and linkage disequilibrium between three loci was found to be significant after applying Bonferroni correction (P < 0.005). These newly developed markers should be significant in population genetic assessment studies or construction of genetic linkage map for this important snakehead fish species in future.

Keywords

Channa argus Microsatellites Simple sequence repeats Genomic library 

Notes

Acknowledgments

This work is sponsored by the Higher Education Commission (HEC), Pakistan and earmarked fund for Modern Agro-industry Technology Research System—Staple Freshwater Fishes Industry Technology System (no. nycytx- 49-03).

References

  1. Brown J, Hardwick LJ, Wright AF (1995) A simple method for rapid isolation of microsatellite from yeast artificial chromosomes. Mol Cell Probes 9:53–58CrossRefPubMedGoogle Scholar
  2. Courtenay WR Jr, Williams JD (2004) Snakeheads (Pisces, Channidae): a biological synopsis and risk assessment. US Depatment of the interior, US Geological survey, Gainsville, p 1251Google Scholar
  3. Excoffier L, Laval G, Schneider S (2005) Arlequin ver 3.1: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  4. Liu J, Cui Y, Liu J (2000) Resting metabolism and heat increment of feeding in mandarin fish (Siniperca chuatsi) and Chinese snakehead (Channa argus). Comp Biochem Physiol 127:131–138CrossRefGoogle Scholar
  5. Lu F, Liu D, Ye X, Wei Y, Liu F (2009) Alginate–calcium coating incorporating nisin and EDTA maintains the quality of fresh northern snakehead (Channa argus) fillets stored at 4°C. J Sci Food Agric 89:848–854CrossRefGoogle Scholar
  6. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225. doi: 10.2307/2409177 CrossRefGoogle Scholar
  7. Rozen S, Skaletsky HJ (2000) Primer 3.0. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: method in molecular biology. Humana press, New Jersey, pp 365–386Google Scholar
  8. Ruihua D (1994) The fishes of Sichuan, China. Sichuan Publishing House of Science and Technology, ChengduGoogle Scholar
  9. Sambrook J, Russel DW (2002) Molecular cloning: a laboratory manual, 3rd edn (trans by Huang P-T et al). Science Press, BeijingGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.College of Fisheries, Key Lab of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhanChina

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