The isolation and characterization of 10 dinucleotide microsatellite markers from enriched Channa argus genomic library
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Eleven microsatellite markers were isolated and characterized from enriched genomic libraries of Channa argus. The loci were scored in 40 specimens from one natural population. We explored 6–17 alleles per locus with observed and expected heterozygosities ranging from 0.8000 to 1.0000 and 0.7269 to 0.9551, respectively, containing 0.830 average polymorphic information content. Significant deviations from Hardy–Weinberg equilibrium were detected only at one loci and linkage disequilibrium between three loci was found to be significant after applying Bonferroni correction (P < 0.005). These newly developed markers should be significant in population genetic assessment studies or construction of genetic linkage map for this important snakehead fish species in future.
KeywordsChanna argus Microsatellites Simple sequence repeats Genomic library
This work is sponsored by the Higher Education Commission (HEC), Pakistan and earmarked fund for Modern Agro-industry Technology Research System—Staple Freshwater Fishes Industry Technology System (no. nycytx- 49-03).
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