Development of 20 microsatellite loci in the Japanese mitten crab Eriocheir japonica and cross-amplification in the Chinese mitten crab Eriocheir sinensis
- 172 Downloads
The Japanese mitten crab (Eriocheir japonica) is a catadromous species endemic to East Asia with the widest distribution of all species in the mitten crab taxonomy. To date, no novel microsatellite loci for this species have been reported. Twenty polymorphic microsatellite loci were isolated, and cross-amplification was conducted in the Yangtze and Yellow River populations of Chinese mitten crab (Eriocheir sinensis). Four loci were found to deviate significantly from the Hardy–Weinberg equilibrium. Similar numbers of alleles, mean observed (H O) and expected (H E) heterozygosities, mean gene diversity, and inbreeding coefficients (F IS) were detected in the two populations of E. sinensis. We concluded that this suite of microsatellite loci was successful for cross-amplification in E. sinensis and might be useful for the assessment of population structure of the mitten crab.
KeywordsMicrosatellite locus Eriocheir japonica Eriocheir sinensis
The authors would like to thank Qinling Yang and Qin Chen for their kind assistance in performing the experiment. The authors give special thanks to Prof. Terry A. Dick (University of Manitoba, Canada) for his kind comments and polish on the manuscript. This work was supported by the Shanghai Science and Technology Project (grant no. 09391911100), the biological breeding project of National Development and Reform Commission (grant no. 2008–2223), and the Shanghai Leading Academic Discipline Project (grant no. Y1101).
- Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86(6):485–486Google Scholar
- Guo JY, Ng NK, Dai A, Ng PKL (1997) The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir de Hann, 1835 (Crustacea: Decapod: Brachyura: Grapsidae). Raffles Bull Zool 45:445–476Google Scholar
- Sakai T (1935) Crustacea of Jehol. (Brachyura) (Order Decapoda). In: Report of the first scientific expedition to Manchoukuo, section V, division 1, part II, article, pp 1–16Google Scholar
- Sakai T (1976) Crabs of Japan and adjacent seas. Kodansha, TokyoGoogle Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York, pp 479–483Google Scholar