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Conservation Genetics Resources

, Volume 2, Supplement 1, pp 47–50 | Cite as

Development of 20 microsatellite loci in the Japanese mitten crab Eriocheir japonica and cross-amplification in the Chinese mitten crab Eriocheir sinensis

  • Qixuan Cheng
  • Chunhong Yuan
  • Jun Wang
  • Jiawei Xu
  • Tai-Hung Lee
  • Chenghui WangEmail author
Technical Note

Abstract

The Japanese mitten crab (Eriocheir japonica) is a catadromous species endemic to East Asia with the widest distribution of all species in the mitten crab taxonomy. To date, no novel microsatellite loci for this species have been reported. Twenty polymorphic microsatellite loci were isolated, and cross-amplification was conducted in the Yangtze and Yellow River populations of Chinese mitten crab (Eriocheir sinensis). Four loci were found to deviate significantly from the Hardy–Weinberg equilibrium. Similar numbers of alleles, mean observed (H O) and expected (H E) heterozygosities, mean gene diversity, and inbreeding coefficients (F IS) were detected in the two populations of E. sinensis. We concluded that this suite of microsatellite loci was successful for cross-amplification in E. sinensis and might be useful for the assessment of population structure of the mitten crab.

Keywords

Microsatellite locus Eriocheir japonica Eriocheir sinensis 

Notes

Acknowledgments

The authors would like to thank Qinling Yang and Qin Chen for their kind assistance in performing the experiment. The authors give special thanks to Prof. Terry A. Dick (University of Manitoba, Canada) for his kind comments and polish on the manuscript. This work was supported by the Shanghai Science and Technology Project (grant no. 09391911100), the biological breeding project of National Development and Reform Commission (grant no. 2008–2223), and the Shanghai Leading Academic Discipline Project (grant no. Y1101).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Qixuan Cheng
    • 1
  • Chunhong Yuan
    • 2
    • 3
  • Jun Wang
    • 1
  • Jiawei Xu
    • 1
  • Tai-Hung Lee
    • 3
  • Chenghui Wang
    • 1
    Email author
  1. 1.Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of AgricultureShanghai Ocean UniversityShanghaiChina
  2. 2.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiChina
  3. 3.Laboratory of Aquaculture Genetics and Genomics, Graduate School of Fisheries SciencesHokkaido UniversityHokkaidoJapan

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