Isolation and characterization of 61 microsatellite markers from sea urchin Strongylocentrotus nudus
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In this study, 61 microsatellite markers were isolated and characterized for sea urchin Strongylocentrotus nudus using the method of screening SSR enriched library. The polymorphism of these markers was assessed using 48 individuals. Number of alleles per locus ranged from two to six. The observed and expected heterozygosities ranged from 0.2340 to 0.8696 and from 0.1304 to 0.7600, respectively. The polymorphic markers developed will be applied to population genetic analysis.
KeywordsMicrosatellite markers Polymorphic Sea urchin Strongylocentrotus nudus
This work was supported by grants from State 863 High-Technology R&D Project of China (2006AA10A411).
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