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Conservation Genetics Resources

, Volume 2, Supplement 1, pp 35–38 | Cite as

Isolation and characterization of 61 microsatellite markers from sea urchin Strongylocentrotus nudus

  • Jingjing Yan
  • Wei Peng
  • Huixia Du
  • Ling Li
  • Zunchun Zhou
  • Zhenmin Bao
  • Jingjie HuEmail author
Technical Note
  • 93 Downloads

Abstract

In this study, 61 microsatellite markers were isolated and characterized for sea urchin Strongylocentrotus nudus using the method of screening SSR enriched library. The polymorphism of these markers was assessed using 48 individuals. Number of alleles per locus ranged from two to six. The observed and expected heterozygosities ranged from 0.2340 to 0.8696 and from 0.1304 to 0.7600, respectively. The polymorphic markers developed will be applied to population genetic analysis.

Keywords

Microsatellite markers Polymorphic Sea urchin Strongylocentrotus nudus 

Notes

Acknowledgments

This work was supported by grants from State 863 High-Technology R&D Project of China (2006AA10A411).

References

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Jingjing Yan
    • 1
  • Wei Peng
    • 1
  • Huixia Du
    • 1
  • Ling Li
    • 1
  • Zunchun Zhou
    • 2
  • Zhenmin Bao
    • 1
  • Jingjie Hu
    • 1
    Email author
  1. 1.Laboratory of Marine Genetics and Breeding, Ministry of EducationOcean University of ChinaQingdaoChina
  2. 2.Liaoning Key Lab of Marine Fishery Molecular BiologyLiaoning Ocean and Fisheries Science Research InstituteDalianChina

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