Development of Fifty-one novel EST-SSR loci in the Pacific oyster, Crassostrea gigas by data mining from the public EST database
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Fifty-one novel EST-derived microsatellite markers were developed from 23,803 expressed sequence tags in the Pacific oyster, Crassostrea gigas. Polymorphisms of these loci were evaluated in a population of 48 individuals. The allele number ranges from 3 to 11, averaging 5.9 alleles per locus. The observed and expected heterozygosity values range from 0.023 to 0.875 (averaging 0.412) and 0.239 to 0.978 (averaging 0.681), respectively. Twenty-one of the 51 markers accord with Hardy–Weinberg equilibrium (HWE), and the rest show significant departure from HWE after Bonferroni correction, apparently due to heterozygote deficiency, suggesting likely occurrence of null alleles at loci and/or subpopulation of the sample. Cross-amplification was examined in C. hongkongensis and C. arikensis and the results show that 23 and 18 loci, respectively, have a reasonable polymorphism in the two species. These EST-SSR markers will be useful in studies of population genetics, linkage mapping and other aspects for this species.
KeywordsCrassostrea gigas EST-SSR Development Polymorphism Cross-species amplification
This work was financially supported by a 863 Program from Ministry of Sciences and Technology of China (No. 2006AA10A409), a Natural Science Foundation of China (no. 30570242) and a Science Foundation of Guangdong Province (8251030101000001). The authors thank Xiaocui He and Rui Ren for their technical assistance and sample collection.
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