Isolation and characterization of polymorphic microsatellite markers for genetic analysis of chub mackerel (Scomber japonicus)
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Chub mackerel, Scomber japonicus, is a species of major importance to fisheries, particularly in eastern Asia. To aid in the sustainable management of this fisheries resource, we isolated eight novel microsatellite loci from the fish and examined their polymorphisms to estimate genetic variability. Genetic variability differed at each locus. The number of alleles ranged from 5 to 14, and observed and expected heterozygosity ranged from 0.43 to 1.00 and 0.50 to 0.86, respectively. One locus showed significant Hardy–Weinberg disequilibrium. No linkage disequilibrium was found. The high variability revealed in this study suggests that these microsatellite markers could prove to be a useful tool for further conservation genetics studies.
KeywordsChub mackerel Genetic marker Microsatellite Scomber japonicus
We thank Dr. J. K. Kim for providing the samples. This work is funded by a grant from the National Fisheries Research and Development (NFRDI) and is contribution number, RP-09-BT-. Views expressed herein are those of the authors and do not necessarily reflect the views of NFRDI.
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