Abstract
We developed 12 microsatellite loci primers in the corkwing wrasse (Symphodus melops). All markers were obtained from partial genomic DNA libraries enriched for tetranucleotide repeats and characterized in 32 unrelated individuals from one putative population. The number of alleles ranged from 5 to 18, with an average of 8.6 per locus, and the observed heterozygosity ranged from 0.464 to 0.969 (average 0.697). Cross-amplification in two closely related commercially exploited species, the ballian wrasse (Labrus bergylta) and the goldsinny wrasse (Ctenolabrus rupestris), successfully resolved four loci of which two were polymorphic and two where monomorphic.
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Acknowledgments
This work was supported by the Norwegian Research Council (through proposal no. 189570/S40) and through the European Science Foundation project “Marine phylogeographic structuring during climate change: the signature of leading and rear edge of range shifting populations” see http://biocongroup.eu/MarinEra/Welcome.html). We thank Kate Enersen for technical assistance in the lab.
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Knutsen, H., Sannæs, H. Development of twelve microsatellite loci in the corkwing wrasse (Symphodus melops). Conservation Genet Resour 1, 433–436 (2009). https://doi.org/10.1007/s12686-009-9100-1
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DOI: https://doi.org/10.1007/s12686-009-9100-1