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Mogurnda adspersa microsatellite markers: multiplexing and multi-tailed primer tagging

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Abstract

A set of twelve microsatellite DNA loci were developed for the threatened Australian freshwater fish Mogurnda adspersa (Eleotridae). Primers were tailed with one of four 20-mer oligonucleotides for use in four-colour fluorescent detection and optimised for multiplex PCR. The loci were used to genotype individuals from two populations in the Pioneer River catchment of central Queensland, eastern Australia. Number of alleles per locus ranged from 2 to 33 and per locus heterozygosity ranged from 0.06 to 0.81. Successful cross-species amplification of all loci was achieved in the congener M. mogurnda. These markers will be used to estimate effective population size and to examine the relationship between flow regime and population demographic parameters.

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Acknowledgements

This work is part of a collaborative project between Griffith University and Queensland Department of Environment and Resource Management (DERM). We thank Steve Smith for refining the protocol. Bernie Cockayne and Kate Engeldow collected fish samples, Ben Cook provided M. mogurnda samples and Jake Butwell constructed laboratory equipment. Australian Animal Ethics Committee approval number for this project is ENV/08/09/AEC. Funding was provided by DERM as part of its Environmental Flows Assessment Program.

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Correspondence to Kathryn M. Real.

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Real, K.M., Schmidt, D.J. & Hughes, J.M. Mogurnda adspersa microsatellite markers: multiplexing and multi-tailed primer tagging. Conservation Genet Resour 1, 411–414 (2009). https://doi.org/10.1007/s12686-009-9095-7

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  • DOI: https://doi.org/10.1007/s12686-009-9095-7

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