Abstract
Seventeen polymorphic microsatellite loci have been isolated from an (AAAG) n enriched genomic library of Onychostoma sima. These loci were tested against 32 wild captured individuals for polymorphism. The number of observed alleles per locus ranged from 2 to 10. The expected and observed heterozygosity values ranged from 0.25 to 0.96 and from 0.26 to 0.87, respectively. Seven loci showed significant deviation from Hardy–Weinberg Equilibrium and linkage equilibrium were detected in three loci. These polymorphic loci described for O. sima will be valuable as genetic markers for use in population structure, breeding, and conservation studies.
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Acknowledgments
We are grateful to Yichao Zhang, Zhi Yang, Di Zhu, Jiangping Tao, Xiang Wang, and Haitao Zheng for their assistance in wild sampling. This work was supported by research grants from Hydro-biodiversity monitoring at Pengshui hydropower station in Wujiang River (PS/HB011-2006) and Yinpan hydropower station in Wujiang River (WL/HB005-2008).
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Xiong, M., Que, Y., Shi, F. et al. Isolation and characterization of microsatellite loci in Onychostoma sima . Conservation Genet Resour 1, 389 (2009). https://doi.org/10.1007/s12686-009-9090-z
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DOI: https://doi.org/10.1007/s12686-009-9090-z