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Characterization of 14 microsatellite loci in the paired lamprey species Ichthyomyzon unicuspis and I. fossor and cross amplification in four other Ichthyomyzon species

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Abstract

We describe the isolation and development of 14 polymorphic microsatellite loci in silver lamprey (Ichthyomyzon unicuspis). The number of alleles ranged from 2 to 11 (with an average of 5.1 alleles per locus) in 23–37 lampreys and genetic diversity ranged from 0.036 to 0.837. All of these loci were also polymorphic (with 2–10 alleles, average 4.4) in the closely related northern brook lamprey (I. fossor), and at least eight were polymorphic in four other Ichthyomyzon species. These loci will aid in studying the population dynamics of these six Ichthyomyzon species.

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Acknowledgements

We would like to thank Nick Lang at the Field Museum in Chicago and Fraser Neave from the Sea Lamprey Control Centre (Fisheries and Oceans Canada) for collection of samples, and Mark Todd of Genetic Identification Services for library construction and microsatellite identification. This project was supported by funding from the Great Lakes Fisheries Commission.

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  1. Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Craig T. McFarlane & Margaret F. Docker

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  1. Craig T. McFarlane
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  2. Margaret F. Docker
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Correspondence to Craig T. McFarlane.

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McFarlane, C.T., Docker, M.F. Characterization of 14 microsatellite loci in the paired lamprey species Ichthyomyzon unicuspis and I. fossor and cross amplification in four other Ichthyomyzon species. Conservation Genet Resour 1, 377 (2009). https://doi.org/10.1007/s12686-009-9087-7

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