Abstract
Nine microsatellite markers were isolated from Pelteobagrus fulvidraco genomic DNA libraries. These nine novel dinucleotide microsatellite loci will provide markers of sufficient power to detect population structure in P. fulvidraco. Polymorphism was tested using 36 individuals from one natural population. The observed number of alleles ranged from 3 to 8. The ranges of observed and expected heterozygosity were 0.3056–0.9444 and 0.5129–0.8435 respectively, the average of PIC was 0.6296. These values showed that these nine microsatellite loci were suitable for genetic analysis.
This is a preview of subscription content, log in via an institution to check access.
References
Chen HJ, Yue YS, Fan XZ, Zhang CS, Du LX (2003) Analysis of genetic diversity of Shandong indigenous chicken breeds using microsatellite marker. J G G 9:855–860 (in Chinese)
Gardner M, Cooper S, Bull C, Grant W (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304
Glenn T, Schable N (2005) Isolating microsatellite DNA loci. Method Enzymol 395:202–222
Kong LJ, Liu FZ (2007) Experiments on culture yellow catfish. Econ Fish 5:72–78
O’Connell M, Wright M (1997) Microsatellite DNA in fishes. Rev Fish Biol Fish 7:331–363
Rozen S, Skaletsky H (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, New Jersey, pp 365–386
Wang CF, Xie SQ, Zhu XM, Lei W, Yang YX, Liu JK (2006) Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae. Aquaculture 254:554–562
Xiao TY, Zhang XW, Zhang HY, Tang XB, Su JM, Liu Z (2004) RAPD analysis of genetic diversity on genomic DNA among 4 species of Pelteobagrus of Dongting Lake. China Biotech 3:84–89
Yeh F, Boyle T (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157
Acknowledgements
Thanks to Mr. Baixiang Chen and Xiong Hu for their help in collection of fish samples. This research was financially supported by the Science Project of Hubei Province, China (No. 2006AA203A02) and the State Key Technologies R&D Programme (No. 2007BAD37B02).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kong, Y., Guo, B., Xie, C. et al. The isolation via enrichment and characterization of 9 dinucleotide microsatellite markers in Pelteobagrus fulvidraco . Conservation Genet Resour 1, 353 (2009). https://doi.org/10.1007/s12686-009-9081-0
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s12686-009-9081-0