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Conservation Genetics Resources

, Volume 2, Supplement 1, pp 1–3 | Cite as

Isolation and characterization of microsatellite loci in Rhinogobio ventralis

  • Nian Xu
  • Fang Shi
  • Meihua Xiong
  • Yanfu Que
  • Zhong Yang
  • Bin Zhu
  • Jianbo ChangEmail author
Technical Note

Abstract

From (AAAG)n enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.

Keywords

Rhinogobio ventralis Microsatellites Polymorphic loci Population genetics 

Notes

Acknowledgments

We thank Di Zhu, Zhi Yang, Yichao Zhang, Ruiping Feng, Ye Qiao, for their help with fish sampling. This work was supported by the project of hydro-biodiversity monitoring at Pengshui hydropower station on Wujiang River (PS/HB011-2006).

References

  1. Aljanabi SM, Martinez I (1997) Universal and rapid salt-extracted of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25:4692–4693CrossRefPubMedGoogle Scholar
  2. Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acid Res 27:573–580CrossRefPubMedGoogle Scholar
  3. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Human Genet 32:314–331Google Scholar
  4. Chen DQ, Duan XB, Liu SP, Shi WG, Wang B (2002) On the dynamics of fishery resources of the Yangtze River and its management. Acta Hydrobiol Sin 26:685–690Google Scholar
  5. Deng HS, He XF (2005) Study on the biology of ventralis in the Changjiang River. J Southwest Agric Univ (Nat Sci) 27:704–708Google Scholar
  6. Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  7. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  8. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16CrossRefPubMedGoogle Scholar
  9. Zhang X, Gao Y (2004) To design PCR primers with Oligo 6 and Primer Premier 5. China J Bioinf 2:15–18Google Scholar
  10. Zhou QG, He XF (1992) A preliminary study on biology of Rhinogobio ventralis in Wujiang River. Freshw Fish 5:11–14Google Scholar
  11. Zhu B, Liao X, Shao Z, Rosenthal H, Chang J (2005) Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis. Mol Ecol Notes 5:888–892CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Nian Xu
    • 1
    • 2
    • 3
  • Fang Shi
    • 1
    • 2
    • 3
  • Meihua Xiong
    • 2
  • Yanfu Que
    • 2
  • Zhong Yang
    • 2
  • Bin Zhu
    • 2
  • Jianbo Chang
    • 2
    Email author
  1. 1.Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  2. 2.Institute of HydroecologyMinistry of Water Resources and Chinese Academy of SciencesWuhanChina
  3. 3.The Graduate School of the Chinese Academy of SciencesBeijingChina

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