Abstract
We developed 11 microsatellite loci primers in the bathypelagic black scabbardfish (Aphanopus carbo), a novelty for deep-sea fishes. All markers were obtained from partial genomic DNA libraries enriched for tetranucleotide repeats and characterized in 50 unrelated individuals from one putative population. The number of alleles ranged from 5 to 40, with an average of 19.3 per locus, and the observed heterozygosity ranged from 0.387 to 0.961 (average 0.749). Cross-amplification in another closely related commercially exploited deep-sea species intermediate scabbardfish (Aphanopus intermedius) resolved 11 polymorphic loci.
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Acknowledgments
This work was supported by the Norwegian Research Council (through proposal no. 161599/V10) and through the European Science Foundation project DEECON (proposal no. 184178/S40). Additional funding was provided Norwegian Ministry of Fishery and Coastal Affairs and by the MAR-ECO (www.mar-eco.no), a field project under the Census of Marine Life programme. We thank Kate Enersen for technical assistance in the lab. S·S. is a researcher contracted by IMAR/DOP under the “Ciência 2007” recruitment funded by FCT (Foundation for Science and Technology, Portugal) with co-funding of POCI 2010 and FSE. IMAR/DOP is funded through the pluri-annual and programmatic funding scheme as research unit #531 and associate laboratory #9.
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Knutsen, H., Catarino, D., Sannæs, H. et al. Development of eleven microsatellite loci in the deep-sea black scabbardfish (Aphanopus carbo). Conservation Genet Resour 1, 89 (2009). https://doi.org/10.1007/s12686-009-9021-z
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DOI: https://doi.org/10.1007/s12686-009-9021-z