Skip to main content

Advertisement

Log in

Characterization of polymorphic microsatellite markers in Valencia letourneuxi (Valenciidae) and cross-amplification in two other cyprinodontiform species

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Fourteen microsatellite loci were isolated from the critically endangered Corfu toothcarp Valencia letourneuxi using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC) probe. Nine of these loci were polymorphic in a V. letourneuxi population of 20 individuals (Vlychos stream) with mean number of alleles per locus = 2.55 and mean observed heterozygosity = 0.3447. Moreover, some of these 9 microsatellite markers and the remaining five loci, monomorphic in V. letourneuxi, were found to be polymorphic in the Spanish samaruc Valencia hispanica and the Mediterranean toothcarp Aphanius fasciatus. These microsatellite loci are expected to be used for population genetic analysis as well as studying the genetic diversity of this endangered species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Barbieri R, Daoulas Ch, Psarras T, Stoumboudi MT, Economou AN (2000) The biology and ecology of Valencia letourneuxi Sauvage 1880 (Valenciidae)—prospects for conservation. Med Mar Sci 1(2):75–90

    Google Scholar 

  • Barbieri R, Stoumboudi MT, Economou AN (2002) Threatened fishes of the world: Valencia letourneuxi Sauvage, 1880 (Valenciidae). Environ Biol Fish 65:46

    Article  Google Scholar 

  • Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, populations, interactions, CNRS UMR 5000, Université de Montpellier II, Montpellier (France)

  • Bianco PG, Miller RR (1989) First record of Valencia letourneuxi (Sauvage 1880) in Peloponnese (Greece) and remarks on the Mediterranean family Valenciidae (Cyprinodontiformes). Cybium 13:385–387

    Google Scholar 

  • Bianco PG, Nordlie F (2008) The salinity tolerance of Pseudophoxinus stymphalicus (Cyprinidae) and Valencia letourneuxi (Valenciidae) from western Greece suggests a revision of the ecological categories of freshwater fishes. Ital J Zool 75(3):285–293

    Article  Google Scholar 

  • Bianco PG, Ahnelt H, Economidis PS (1996) The freshwater fishes from eastern and large Mediterranean islands with comments on their safety status. Acta Univ Carol Biol 40:45–60

    Google Scholar 

  • Economidis PS (1995) Endangered freshwater fishes of Greece. Biol Conserv 72:201–211

    Article  Google Scholar 

  • Goudet J (1995) Fstat version 1.2: a computer program to calculate F statistics. J Hered 86:485–486

    Google Scholar 

  • Kalogianni E, Giakoumi S, Zogaris S, Barbieri R, Stoumboudi MT, Zimmerman B, Economou AN (2006) Population decline of the endangered fish species Valencia letourneuxi. In: Western Greece—strategies for conservation. 1st European Congress of Conservation Biology, Book of Abstracts, p 520

  • Kottelat M, Freyhof J (2007) Handbook of European freshwater fishes. Kottelat, Cornol, Switzerland and Freyhof, Berlin, Germany (Eds), Kottelat Publ., p 646

  • Maltagliati F, Lai T, Casu M, Valdesalici S, Castelli A (2006) Identification of endangered Mediterranean cyprinodontiform fish by means of DNA inter-simple sequence repeats (ISSRs). Biochem Syst Ecol 34(8):626–634

    Article  CAS  Google Scholar 

  • Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215

    Article  CAS  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

  • Tsigenopoulos CS, Hellemans B, Christiakov DA, Libertini A, Kotoulas G, Völckaert F (2003) Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.). Mol Ecol Notes 3:352–354

    Article  CAS  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank J. Lagnel and I. Magiopoulos for technical assistance and R. Hanel for providing the V. hispanica samples. Financial support was provided by an Excellence Grant to the IMBG (Service Plan 2006–2008) from the Hellenic General Secretariat for Research and Technology (GSRT).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Costas S. Tsigenopoulos.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vogiatzi, E., Kalogianni, E., Giakoumi, S. et al. Characterization of polymorphic microsatellite markers in Valencia letourneuxi (Valenciidae) and cross-amplification in two other cyprinodontiform species. Conservation Genet Resour 1, 27–30 (2009). https://doi.org/10.1007/s12686-009-9006-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-009-9006-y

Keywords

Navigation