Hormones and Cancer

, Volume 6, Issue 2–3, pp 87–99 | Cite as

Characterization of Thyroid Cancer Cell Lines in Murine Orthotopic and Intracardiac Metastasis Models

  • Jennifer A. Morrison
  • Laura A. Pike
  • Greg Lund
  • Qiong Zhou
  • Brittelle E. Kessler
  • Kevin T. Bauerle
  • Sharon B. Sams
  • Bryan R. Haugen
  • Rebecca E. Schweppe
Original Paper


Thyroid cancer incidence has been increasing over time, and it is estimated that ∼1950 advanced thyroid cancer patients will die of their disease in 2015. To combat this disease, an enhanced understanding of thyroid cancer development and progression as well as the development of efficacious, targeted therapies are needed. In vitro and in vivo studies utilizing thyroid cancer cell lines and animal models are critically important to these research efforts. In this report, we detail our studies with a panel of authenticated human anaplastic and papillary thyroid cancer (ATC and PTC) cell lines engineered to express firefly luciferase in two in vivo murine cancer models—an orthotopic thyroid cancer model as well as an intracardiac injection metastasis model. In these models, primary tumor growth in the orthotopic model and the establishment and growth of metastases in the intracardiac injection model are followed in vivo using an IVIS imaging system. In the orthotopic model, the ATC cell lines 8505C and T238 and the PTC cell lines K1/GLAG-66 and BCPAP had take rates >90 % with final tumor volumes ranging 84–214 mm3 over 4–5 weeks. In the intracardiac model, metastasis establishment was successful in the ATC cell lines HTh74, HTh7, 8505C, THJ-16T, and Cal62 with take rates ≥70 %. Only one of the PTC cell lines tested (BCPAP) was successful in the intracardiac model with a take rate of 30 %. These data will be beneficial to inform the choice of cell line and model system for the design of future thyroid cancer studies.


Thyroid Cancer Papillary Thyroid Cancer Thyroid Cancer Cell Anaplastic Thyroid Cancer TERT Promoter Mutation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank Dr. Jeffrey Knauf at Memorial Sloan Kettering Cancer Center for Sequenom analysis of the cell lines, Drs. Jeffrey Myers and Maria Gule at MD Anderson Cancer Center for their guidance in establishing the orthotopic thyroid cancer model, and Dr. Carol Sartorius at the University of Colorado for her guidance in establishing the intracardiac injection model. We also thank Randall Wong at the B. Davis Center BioResources Core Facility, Molecular Biology Unit, and Dr. Christopher Korch, UCCC, for STR profiling of the cell lines. This work was supported by National Cancer Institute grant K12CA086913-13 (RES), 1R01CA164193 (RES), American Cancer Society RSG-13-060-01-TBE (RES), 1 RC1 CA147371 (RES and BRH), and 1R01CA155512-01A1 (BRH). The UCCC Flow Cytometry Core, UCCC Sequencing and Analysis Core (for STR profiling), UCCC Pathology Core, and UCCC Small Animal Imaging Cores are supported by NCI Cancer Center support grant P30 CA046934.

Conflict of Interest


Supplementary material

12672_2015_219_MOESM1_ESM.pptx (2.2 mb)
Supplemental Fig. 1 BCPAP PTC cells form tumors and lung metastases in the orthotopic tumor model. A representative H&E stained primary tumor specimen is shown at ×10 and ×20 magnification (a). Arrow indicates area of normal thyroid follicles. Lung micrometastases were identified in lung tissue harvested from mice at necropsy and are shown at ×20 magnification (b). (PPTX 2266 kb)


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jennifer A. Morrison
    • 1
  • Laura A. Pike
    • 1
  • Greg Lund
    • 1
  • Qiong Zhou
    • 1
  • Brittelle E. Kessler
    • 1
  • Kevin T. Bauerle
    • 1
  • Sharon B. Sams
    • 2
  • Bryan R. Haugen
    • 1
    • 2
  • Rebecca E. Schweppe
    • 1
    • 2
  1. 1.Department of Medicine, Division of Endocrinology, Metabolism and DiabetesUniversity of Colorado Anschutz Medical CampusAuroraUSA
  2. 2.Department of PathologyUniversity of Colorado Anschutz Medical CampusAuroraUSA

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