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Characterization of Green Synthesized Antibacterial Silver Nanoparticles from Amaranthus spinosus L. Extract

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Abstract

This study aimed to use aqueous extracts of Amaranthus spinosus L. leaves as a reducing and stabilizing agent in the green synthesis of silver nanoparticles. Silver nitrate concentrations of 0.1, 1, and 3 mM were used in the biosynthesis of silver nanoparticles. The biosynthesized silver nanoparticles were characterized and the antibacterial efficacy of the synthesized silver nanoparticles was tested against clinical strains of Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus. In UV-Vis spectra, bio-silver nanoparticles had the highest absorbance at 420 nm, with a color change from yellow to reddish brown. The vibrations recorded from the FTIR justify the possible reducing and stabilizing biomolecules of Amaranthus spinosus leaf extract involved in the reduction of silver ions to silver nanoparticles. XRD spectra and SEM revealed the crystallinity and cubic and hexagonal shape of bio-silver nanoparticles, respectively. Bio-silver nanoparticles have got a maximum antibacterial activity of about 19.3 ± 0.5 mm and 21.3 ± 0.5 mm zone of inhibition against Pseudomonas aeruginosa with a lower MIC and MBC of about 0.1 and 1 mg/ml. The leaf extract of Amaranthus spinosus L. was suitable for the green synthesis of silver nanoparticles. These bio-nanoparticles showed remarkable antimicrobial activity against Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus.

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References

  1. Ahmed, S., Saifullah, Ahmad, M., Swami, B. L., & Ikram, S. (2016). Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Science, 9(1), 1–7. https://doi.org/10.1016/j.jrras.2015.06.006

    Article  Google Scholar 

  2. Iravani, S., Korbekandi, H., Mirmohammadi, S. V., & Zolfaghari, B. (2014). Synthesis of silver nanoparticles: Chemical, physical and biological methods. Research in Pharmaceutical Sciences, 9(6), 385–406.

    Google Scholar 

  3. Goudarzi, M., Mir, N., Mousavi-Kamazani, M., Bagheri, S., & Salavati-Niasari, M. (2016). Biosynthesis and characterization of silver nanoparticles prepared from two novel natural precursors by facile thermal decomposition methods. Scientific Reports, 6(1), 1–13. https://doi.org/10.1038/srep32539

    Article  Google Scholar 

  4. Sun, Y., & Xia, Y. (2002). Shape-controlled synthesis of gold and silver nanoparticles. Science., 298(5601), 2176–2179. https://doi.org/10.1126/science.1077229

    Article  Google Scholar 

  5. Kagithoju, S., Godishala, V., & Nanna, R. S. (2015). Eco-friendly and green synthesis of silver nanoparticles using leaf extract of Strychnos potatorum Linn.F. and their bactericidal activities. 3Biotech., 5(5), 709–714. https://doi.org/10.1007/s13205-014-0272-3

    Article  Google Scholar 

  6. Abdelghany, T. M., Al-Rajhi, A. M. H., Al Abboud, M. A., Alawlaqi, M. M., Ganash Magdah, A., Helmy, E. A. M., et al. (2017). Recent advances in green synthesis of silver nanoparticles and their applications: About future directions. A review. BioNanoScience., 8(1), 5–16. https://doi.org/10.1007/s12668-017-0413-3

    Article  Google Scholar 

  7. Sreelekha, E., George, B., Shyam, A., Sajina, N., & Mathew, B. (2021). A comparative study on the synthesis, characterization, and antioxidant activity of green and chemically synthesized silver nanoparticles. BioNanoScience., 11(2), 489–496. https://doi.org/10.1007/s12668-021-00824-7

    Article  Google Scholar 

  8. Shankar, S. S., Rai, A., Ahmad, A., & Sastry, M. (2004). Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. Journal of Colloid and Interface Science, 275(2), 496–502. https://doi.org/10.1016/j.jcis.2004.03.003

    Article  Google Scholar 

  9. Kim, J. S., Kuk, E., Yu, K. N., Kim, J. H., Park, S. J., Lee, H. J., et al. (2007). Antimicrobial effects of silver nanoparticles. Nanomedicine., 3(1), 95–101. https://doi.org/10.1016/j.nano.2006.12.001

    Article  Google Scholar 

  10. Sondi, I., & Salopek-Sondi, B. (2004). Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 275(1), 177–182. https://doi.org/10.1016/j.jcis.2004.02.012

    Article  Google Scholar 

  11. Senguttuvan, J., Paulsamy, S., & Karthika, K. (2014). Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine, 4, S359–S367. https://doi.org/10.12980/apjtb.4.2014c1030

    Article  Google Scholar 

  12. Jain, S., & Mehata, M. S. (2017). Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Scientific Reports, 7(1), 15867. https://doi.org/10.1038/s41598-017-15724-8

    Article  Google Scholar 

  13. Ndikau, M., Noah, N. M., Andala, D. M., & Masika, E. (2017). Green synthesis and characterization of silver nanoparticles using Citrullus lanatus fruit rind extract. International Journal of Analytical Chemistry, 2017, 8108504. https://doi.org/10.1155/2017/8108504

    Article  Google Scholar 

  14. Annamalai, J., & Nallamuthu, T. (2016). Green synthesis of silver nanoparticles: Characterization and determination of antibacterial potency. Applied Nanoscience, 6, 259–265. https://doi.org/10.1007/s13204-015-0426-6

    Article  Google Scholar 

  15. Kumar Sur, U., Ankamwar, B., Karmakar, S., Halder, A., & Das, P. (2018). Green synthesis of silver nanoparticles using the plant extract of Shikakai and Reetha. Materials Today: Proceedings., 5(1), 2321–2329. https://doi.org/10.1016/j.matpr.2017.09.236

    Article  Google Scholar 

  16. Anandalakshmi, K., Venugobal, J., & Ramasamy, V. (2015). Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience, 6(3), 399–408. https://doi.org/10.1007/s13204-015-0449-z

    Article  Google Scholar 

  17. Ahmad, A., Mukherjee, P., Senapati, S., Mandal, D., Khan, M. I., Kumar, R., et al. (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces B: Biointerfaces, 28(4), 313–318. https://doi.org/10.1016/S0927-7765(02)00174-1

    Article  Google Scholar 

  18. Ahmad, N., & Sharma, S. (2012). Green synthesis of silver nanoparticles using extracts of Ananas comosus. Green and Sustainable Chemistry, 02(04), 141–147. https://doi.org/10.4236/gsc.2012.24020

    Article  Google Scholar 

  19. Ajitha, B., Reddy, Y. A., & Reddy, P. S. (2015). Biosynthesis of silver nanoparticles using Momordica charantia leaf broth: Evaluation of their innate antimicrobial and catalytic activities. Journal of Photochemistry and Photobiology. B, 146, 1–9. https://doi.org/10.1016/j.jphotobiol.2015.02.017

    Article  Google Scholar 

  20. Logeswari, P., Silambarasan, S., & Abraham, J. (2015). Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. Journal of Saudi Chemical Society, 19(3), 311–317. https://doi.org/10.1016/j.jscs.2012.04.007

    Article  Google Scholar 

  21. Mohan, S., Oluwafemi, O. S., George, S. C., Jayachandran, V. P., Lewu, F. B., Songca, S. P., et al. (2014). Completely green synthesis of dextrose reduced silver nanoparticles, its antimicrobial and sensing properties. Carbohydrate Polymers, 106, 469–474. https://doi.org/10.1016/j.carbpol.2014.01.008

    Article  Google Scholar 

  22. Ali, Z. A., Yahya, R., Sekaran, S. D., & Puteh, R. (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Advances in Materials Science and Engineering, 2016, 1–6. https://doi.org/10.1155/2016/4102196

    Article  Google Scholar 

  23. Allafchian, A. R., Mirahmadi-Zare, S. Z., Jalali, S. A. H., Hashemi, S. S., & Vahabi, M. R. (2016). Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity. Journal of Nanostructure in Chemistry, 6(2), 129–135. https://doi.org/10.1007/s40097-016-0187-0

    Article  Google Scholar 

  24. Nanda, A., & Saravanan, M. (2009). Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE. Nanomedicine., 5(4), 452–456. https://doi.org/10.1016/j.nano.2009.01.012

    Article  Google Scholar 

  25. Wypij, M., Czarnecka, J., Swiecimska, M., Dahm, H., Rai, M., & Golinska, P. (2018). Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World Journal of Microbiology and Biotechnology, 34(2), 23. https://doi.org/10.1007/s11274-017-2406-3

    Article  Google Scholar 

  26. Ibrahim, H. M. M. (2019). Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. Journal of Radiation Research and Applied Science, 8(3), 265–275. https://doi.org/10.1016/j.jrras.2015.01.007

    Article  Google Scholar 

  27. Akhter, S. M. H., Mohammad, F., & Ahmad, S. (2019). Terminalia belerica mediated green synthesis of nanoparticles of copper, iron and zinc metal oxides as the alternate antibacterial agents against some common pathogens. BioNanoScience., 9(2), 365–372. https://doi.org/10.1007/s12668-019-0601-4

    Article  Google Scholar 

  28. Golabiazar, R., Othman, K. I., Khalid, K. M., Maruf, D. H., Aulla, S. M., & Yusif, P. A. (2019). Green synthesis, characterization, and investigation antibacterial activity of silver nanoparticles using Pistacia atlantica leaf extract. BioNanoScience., 9(2), 323–333. https://doi.org/10.1007/s12668-019-0606-z

    Article  Google Scholar 

  29. Peter Amaladhas, T., Sivagami, S., Akkini Devi, T., Ananthi, N., & Priya Velammal, S. (2012). Biogenic synthesis of silver nanoparticles by leaf extract of Cassia angustifolia. Advances in Natural Sciences: Nanoscience and Nanotechnology, 3(4), 045006. https://doi.org/10.1088/2043-6262/3/4/045006

    Article  Google Scholar 

  30. Chan, Y. S., & Mat Don, M. (2012). Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities. The International Arabic Journal of Antimicrobial Agents, 2(3), 1–8.

    Google Scholar 

  31. Theivasanthi, T., & Alagar, M. (2012). Electrolytic synthesis and characterization of silver nanopowder. Nano Biomedicine and Engineering, 4(2), 58–65. https://doi.org/10.5101/nbe.v4i2.p58-65

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to STIC, Cochin for SEM analysis and thankful to the Professors, Researchers, and Students of the Department of Life Sciences University of Calicut for their generous support for completing this research work.

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Both authors worked together to complete this project. The study was conceptualized and designed by author Denoj Sebastian. Author Preenanka R conducted the experiments, analyzed the data, drafted the first draft of the manuscript, and oversaw the literature searches. The final draft was revised and proofread by author Denoj Sebastian. The final manuscript was read and approved by both authors.

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Correspondence to Denoj Sebastian.

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R, P., Sebastian, D. Characterization of Green Synthesized Antibacterial Silver Nanoparticles from Amaranthus spinosus L. Extract. BioNanoSci. 12, 502–511 (2022). https://doi.org/10.1007/s12668-022-00965-3

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