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Characterization of Green Synthesized Antibacterial Silver Nanoparticles from Amaranthus spinosus L. Extract

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This study aimed to use aqueous extracts of Amaranthus spinosus L. leaves as a reducing and stabilizing agent in the green synthesis of silver nanoparticles. Silver nitrate concentrations of 0.1, 1, and 3 mM were used in the biosynthesis of silver nanoparticles. The biosynthesized silver nanoparticles were characterized and the antibacterial efficacy of the synthesized silver nanoparticles was tested against clinical strains of Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus. In UV-Vis spectra, bio-silver nanoparticles had the highest absorbance at 420 nm, with a color change from yellow to reddish brown. The vibrations recorded from the FTIR justify the possible reducing and stabilizing biomolecules of Amaranthus spinosus leaf extract involved in the reduction of silver ions to silver nanoparticles. XRD spectra and SEM revealed the crystallinity and cubic and hexagonal shape of bio-silver nanoparticles, respectively. Bio-silver nanoparticles have got a maximum antibacterial activity of about 19.3 ± 0.5 mm and 21.3 ± 0.5 mm zone of inhibition against Pseudomonas aeruginosa with a lower MIC and MBC of about 0.1 and 1 mg/ml. The leaf extract of Amaranthus spinosus L. was suitable for the green synthesis of silver nanoparticles. These bio-nanoparticles showed remarkable antimicrobial activity against Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus.

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The authors are grateful to STIC, Cochin for SEM analysis and thankful to the Professors, Researchers, and Students of the Department of Life Sciences University of Calicut for their generous support for completing this research work.

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Both authors worked together to complete this project. The study was conceptualized and designed by author Denoj Sebastian. Author Preenanka R conducted the experiments, analyzed the data, drafted the first draft of the manuscript, and oversaw the literature searches. The final draft was revised and proofread by author Denoj Sebastian. The final manuscript was read and approved by both authors.

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Correspondence to Denoj Sebastian.

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R, P., Sebastian, D. Characterization of Green Synthesized Antibacterial Silver Nanoparticles from Amaranthus spinosus L. Extract. BioNanoSci. 12, 502–511 (2022).

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