Skip to main content

Advertisement

SpringerLink
Log in

Preparation and Characterization of Nano-Lipopeptide Biosurfactant Hydrogel and Evaluation of Wound-Healing Properties

  • Published:
BioNanoScience Aims and scope Submit manuscript

Abstract

Nano-formulation of biosurfactant can enhance the absorption of active material. The aim of this study was to produce a nano-lipopeptide biosurfactant (NLPB) formulation from lipopeptide biosurfactant (LPB) produced by Acinetobacter junii, which was previously isolated from oil exploration areas. Besides, the wound-healing activity of the obtained NLPB formulation was also determined. The NLPB was prepared using reflux method with microwave irradiation and loaded on carbomer hydrogel formulation. The physicochemical and structural properties of NLPB formulation were evaluated using dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). For determination of in vivo wound-healing activity, the prepared formulation was administered to 24 rats (previously wounded in depilated thoracic region) which were randomly distributed into six groups. The examined parameters for evaluation of the wound-healing activity were percentage wound contraction and histopathological study of regenerated tissue by hematoxylin and eosin (H&E) and trichrome staining. The size of the LPB was decreased to 80 nm by reflux method. The NLPB formulation had the most stable physicochemical properties. For the in vivo test, the concentration of 2 mg/ml was selected as the best formulation. In vivo test on rats also showed the best efficacy of the formulation on wound closure and healing. NLPB has the same physical properties as the LPB. Moreover, by decreasing the size of LPB, the wound-healing property of the LPB increased.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Data Availability

The analyzed datasets generated during the current study are available from the corresponding author on reasonable request.

Abbreviations

NLPB:

Nano-lipopeptide biosurfactant

LPB:

Lipopeptide biosurfactant

DLS:

Dynamic light scattering

FT-IR:

Fourier transform infrared spectroscopy

SEM:

Scanning electron microscopy

H&E:

Hemotoxylin and eosin

References

  1. Razali, M. H., Ismail, N. A., & Amin, K. A. M. (2019). Titanium dioxide nanotubes incorporated gellan gum bio-nanocomposite film for wound healing: Effect of TiO2 nanotubes concentration. International Journal of Biological Macromolecules.

  2. Kakakhel, M.A., et al. (2021). Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish. Microscopy Research and Technique.

  3. Alshubaily, F. A., & Al-Zahrani, M. H. (2019). Appliance of fungal chitosan/ceftriaxone nano-composite to strengthen and sustain their antimicrobial potentiality against drug resistant bacteria. International Journal of Biological Macromolecules, 135, 1246–1251.

    Article  Google Scholar 

  4. Onaizi, S.A. (2021). Statistical Analyses of the effect of rhamnolipid biosurfactant addition on the enzymatic removal of bisphenol A from wastewater. Biocatalysis and Agricultural Biotechnology. p. 101929.

  5. Akbari, S., et al. (2018). Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology Research and Innovation, 2(1), 81–90.

    Article  MathSciNet  Google Scholar 

  6. Santos, D. K., et al. (2013). Synthesis and evaluation of biosurfactant produced by Candida lipolytica using animal fat and corn steep liquor. Journal of Petroleum Science and Engineering, 105, 43–50.

    Article  Google Scholar 

  7. Satpute, S. K., et al. (2010). Methods for investigating biosurfactants and bioemulsifiers: A review. Critical Reviews in Biotechnology, 30(2), 127–144.

    Article  Google Scholar 

  8. Ohadi, M., et al. (2018). Investigation of the structural, physicochemical properties, and aggregation behavior of lipopeptide biosurfactant produced by Acinetobacter junii B6. International Journal of Biological Macromolecules, 112, 712–719.

    Article  Google Scholar 

  9. Singh, P., Patil, Y., & Rale, V. (2019). Biosurfactant production: Emerging trends and promising strategies. Journal of Applied Microbiology, 126(1), 2–13.

    Article  Google Scholar 

  10. Yea, D., Jo, S., & Lim, J. (2019). Synthesis of Eco-friendly nano-structured biosurfactants from vegetable oil sources and characterization of their interfacial properties for cosmetic applications. MRS Advances, 4(7), 377–384.

    Article  Google Scholar 

  11. Kashapov, R. R., et al. (2019). Self-assembling and biological properties of single-chain dicationic pyridinium-based surfactants. Colloids and Surfaces B: Biointerfaces, 175, 351–357.

    Article  Google Scholar 

  12. Ohadi, M., et al. (2020). Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6. Microbial Pathogenesis, 138, 103806.

    Article  Google Scholar 

  13. Saeedi, L. H., et al. (2014). The production and evaluation of a nano-biosurfactant. Petroleum Science and Technology, 32(2), 125–132.

    Article  Google Scholar 

  14. Foad, A.F.A. (2020). Acute and chronic wound healing physiology, in Diabetic Foot Ulcer. Springer p. 63–76.

  15. Sun, M., et al. (2020). Preparation and characterization of epigallocatechin gallate, ascorbic acid, gelatin, chitosan nanoparticles and their beneficial effect on wound healing of diabetic mice. International Journal of Biological Macromolecules

  16. Ohadi, M., et al. (2017). Antioxidant potential and wound healing activity of biosurfactant produced by Acinetobacter junii B6. Current Pharmaceutical Biotechnology, 18(11), 900–908.

    Article  MathSciNet  Google Scholar 

  17. Ohadi, M., et al. (2017). Isolation, characterization, and optimization of biosurfactant production by an oil-degrading Acinetobacter junii B6 isolated from an Iranian oil excavation site. Biocatalysis and Agricultural Biotechnology, 12, 1–9.

    Article  Google Scholar 

  18. Karimi, M. A., et al. (2020). Microwave-assisted synthesis of the Fe2O3/g-C3N4 nanocomposites with enhanced photocatalytic activity for degradation of methylene blue. Journal of the Chinese Chemical Society, 67(11), 2032–2041.

    Article  Google Scholar 

  19. Szekalska, M., et al. (2020). In vivo anti-inflammatory and anti-allergic activities of cynaroside evaluated by using hydrogel formulations. Biomedicine & Pharmacotherapy, 121, 109681.

    Article  Google Scholar 

  20. Ohadi, M., et al. (2020). Biosynthesis of gold nanoparticles assisted by lipopeptide biosurfactant derived from Acinetobacter junii B6 and evaluation of its antibacterial and cytotoxic activities. BioNanoScience, 10(4), 899–908.

    Article  Google Scholar 

  21. Ravindran, A., et al. (2020). Revealing the efficacy of thermostable biosurfactant in heavy metal bioremediation and surface treatment in vegetables. Frontiers in Microbiology, 11, 222.

    Article  Google Scholar 

  22. Gil-Font, J., et al. (2020). Improving heat transfer of stabilised thermal oil-based tin nanofluids using biosurfactant and molecular layer deposition. Applied Thermal Engineering, 178, 115559.

    Article  Google Scholar 

  23. Natarajan, V., et al. (2013). Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101(4), 560–567.

    Article  Google Scholar 

  24. Xue, M., & Jackson, C. J. (2015). Extracellular matrix reorganization during wound healing and its impact on abnormal scarring. Advances in Wound Care, 4(3), 119–136.

    Article  Google Scholar 

  25. Ismail, W., et al. (2013). Characterization of a lipopeptide biosurfactant produced by a crude-oil-emulsifying Bacillus sp. I-15. International Biodeterioration & Biodegradation, 84, 168–178.

    Article  Google Scholar 

  26. Sánchez, S., Soler, L., & Katuri, J. (2015). Chemically powered micro-and nanomotors. Angewandte Chemie International Edition, 54(5), 1414–1444.

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the council of Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Author information

Authors and Affiliations

  1. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Sepehr Afsharipour, Alireza Asadi, Mandana Ohadi, Mehdi Ranjbar & Gholamreza Dehghannoudeh

  2. Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Hamid Forootanfar

  3. Department of Pathology and Stem Cell Research, Kerman University of Medical Sciences, Kerman, Iran

    Elham Jafari

  4. Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Scences, Kerman, Iran

    Gholamreza Dehghannoudeh

Authors
  1. Sepehr Afsharipour
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alireza Asadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mandana Ohadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mehdi Ranjbar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hamid Forootanfar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elham Jafari
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gholamreza Dehghannoudeh
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Sepehr Afsharipour, Gholamreza Dehghannoudeh, Mehdi Ranjbar, Hamid Forootanfar, Alireza Asadi, and Mandana Ohadi had significant involvement in the design, acquisition, analysis, and interpretation of the data. Ibrahim M. Banat and Elham Jafari were significantly provided guidance in the overall design and delivery of the research. All authors were involved in revising the content, agree to take accountability for the integrity and accuracy of the work, and have read and approved the final manuscript.

Corresponding author

Correspondence to Gholamreza Dehghannoudeh.

Ethics declarations

Conflict of Interest

The authors declare no competing interests.

Ethics Approval and Consent to Participate

The study was approved by the Kerman University of Medical Sciences Ethics Committee as certification code no. 91837723842.

Consent for Publication

Not applicable

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Afsharipour, S., Asadi, A., Ohadi, M. et al. Preparation and Characterization of Nano-Lipopeptide Biosurfactant Hydrogel and Evaluation of Wound-Healing Properties. BioNanoSci. 11, 1061–1069 (2021). https://doi.org/10.1007/s12668-021-00896-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12668-021-00896-5

Keywords

Search

Navigation