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Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver Nanoparticles from Stem Bark of Terminalia brownii

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Abstract

The present study focused on synthesizing, characterization, and evaluating an in vitro anti-inflammatory and cytotoxic potential of green synthesized silver nanoparticles (TB-Ag NPs) from aqueous stem bark extract of Terminalia brownii (TB-AQ). The TB-Ag NPs were characterized by ultraviolet (UV)-visible, Fourier-transform infrared spectroscopy (FTIR), dynamic light X-ray diffraction, and energy-dispersive X-ray spectroscopy, as well as scanning and transmission electron microscopy. The in vitro anti-inflammatory and cytotoxic potential of TB-Ag NPs and TB-AQ were evaluated against RAW 264.7 macrophage and MDA-MB-231 triple-negative breast cancer cells, respectively, by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the inhibitory effect on LPS-induced production of inflammatory mediators PGE2 and NO in RAW 264.7 cell lines was evaluated. The results showed that TB-Ag NPs were crystalline with face-centered spherical polydispersed shaped nanoparticles with an average size between 20 and 67 ± 0.5 nm. Also, TB-Ag NPs had no cytotoxic effect on RAW cells (normal healthy cells) in the range of 6.25–50 µg/mL. Besides, TB-Ag NPs at 50 µg/mL concentration exhibited 67.02% of cytotoxic effect against MDA-MB-231 cells with observed IC50 values of 29.08 µg/mL. TB-Ag NPs have shown significantly in vitro anti-inflammatory activities by exhibiting dose-dependent NO and PGE2 inhibitory activities with IC50 values of 32.82 µg/mL and 67.25 µg/mL, respectively. This study concluded that the novel green synthesized TB-Ag NPs can be used as a potential novel anti-inflammatory and cytotoxicity agent to treat inflammatory-related diseases and inflammatory breast cancer (invasive ductal carcinoma) with biocompatible nature by targeting the tumor environment.

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Acknowledgements

We are thankful to the office of Research and Post Graduate Studies Directorate, Aksum University, Axum, Ethiopia, for providing us financial support in the form of a student project. We extend our deepest thanks to the Department of Nanotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, for characterization studies and Cancer Biology Lab, Department of Biochemistry, and Bioinformatics, GITAM University, Visakhapatnam, Andhra Pradesh, India, to support us to carry out anticancer activities.

Funding

This project was funded by the Post Graduate Research Directorate, Aksum University, Axum, Ethiopia, for providing financial support in the form of a Post Graduate Student project.

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  1. Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Post Box 1010, Axum, Tigray Region, Ethiopia

    Haftamu Tesfay Berihu, Tesfay Welderfael, Berihu Tekluu, V. K. Gopalakrishnan & K. Krishna Chaithanya

  2. Cancer Biology Lab, Department of Biochemistry and Bioinformatics, GITAM (Deemed To Be University), Visakhapatnam, 530045, Andhra Pradesh, India

    M. Rama Rao

  3. Department of Zoology, College of Science and Technology, Andhra University, Visakhapatnam, 530003, Andhra Pradesh, India

    P. P. N. Vijay Kumar & U. Shameem

  4. Research and Development, Centre for Computational and Biological Sciences, Dommeru, West Godavari, 534350, Andhra Pradesh, India

    P. John Dogulas

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Berihu, H.T., Welderfael, T., Tekluu, B. et al. Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver Nanoparticles from Stem Bark of Terminalia brownii. BioNanoSci. 11, 998–1016 (2021). https://doi.org/10.1007/s12668-021-00885-8

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