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Antibacterial Studies of ZnO and Cu-Doped ZnO Nanoparticles Synthesized Using Aqueous Leaf Extract of Stachytarpheta jamaicensis

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Abstract

Zinc oxide (ZnO) and Cu-doped ZnO nanoparticles (1% and 5%; Cu-doped ZnO NPs) were synthesized using aqueous leaf extract of Stachytarpheta jamaicensis. Crystalline ZnO and Cu-doped ZnO NPs with hexagonal wurtzite structure were obtained without any impurities as confirmed by powder X-ray diffraction. The optical properties, morphology, elemental composition, and surface analysis of all the samples were studied using UV-vis diffuse reflectance spectroscopy, scanning electron microscopy equipped with energy dispersive X-ray analysis (SEM-EDX), and X-ray photoelectron spectroscopy, respectively. Antibacterial activities of the ZnO and Cu-doped ZnO were performed by screening test at the highest concentration (500 mg/mL) against two gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus) and two gram-negative bacterial strains (Pseudomonas aeruginosa and Escherichia coli). Antibacterial activities were observed only against gram-positive bacteria. Minimum inhibitory concentration (MIC) ranged from < 25 to 50 mg/mL.

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Acknowledgments

Authors would like to acknowledge the FIC grant UBD/RSCH/1.4/FICBF(b)/2018/012 from Universiti Brunei Darussalam, Brunei Darussalam.

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Authors and Affiliations

  1. Chemical Sciences, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE, 1410, Brunei Darussalam

    Mohammad Mansoob Khan, Mohammad Hilni Harunsani & Ai Ling Tan

  2. Institut für Chemie, Technische Universität Berlin, Straße des 17, Juni 135, 10623, Berlin, Germany

    Mirabbos Hojamberdiev

  3. Environmental and Life Sciences, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE, 1410, Brunei Darussalam

    Yap Ai Poi & Norhayati Ahmad

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  1. Mohammad Mansoob Khan
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  2. Mohammad Hilni Harunsani
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Khan, M.M., Harunsani, M.H., Tan, A.L. et al. Antibacterial Studies of ZnO and Cu-Doped ZnO Nanoparticles Synthesized Using Aqueous Leaf Extract of Stachytarpheta jamaicensis. BioNanoSci. 10, 1037–1048 (2020). https://doi.org/10.1007/s12668-020-00775-5

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