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Synthesis and Apoptotic Efficacy of Biosynthesized Silver Nanoparticles Using Acacia luciana Flower Extract in MCF-7 Breast Cancer Cells: Activation of Bak1 and Bclx for Cancer Therapy

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Abstract

Due to unique properties of green silver nanoparticles, especially chemical and biological properties, they are one of the most used metallic nanoparticles in modern healthcare applications. Accordingly, we synthesized Ag-NPs using Acacia luciana as a reducing and stabilizing agent, to elucidate its apoptosis inducing ability in MCF-7 cancer cells. The Ag-NPs were characterized using FTIR and FESEM. These instruments confirmed that biosynthesized nanoparticles are rod-shaped with average size of 50 nm and negative surface charge of 2.59 mV. The in vitro anticancer effects using MTT assay revealed that the Ag-NPs showed inhibition of cell survival in a time and dose-dependent manner with IC50 value of 4.37 mg/mL. Also, the expression of pro- and antiapoptotic genes was measured using real-time PCR. The results hinted that Ag-NPs can significantly increase the Bak1/Bclx ratios through the change in mitochondrial membrane permeability and be used as a therapeutic agent breast cancer cells.

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Acknowledgments

The authors thank the Deputy of Research and Technology of Zabol University of Medical Sciences for all support provided.

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Authors and Affiliations

  1. Department of Medicinal Chemistry, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

    Azam Sargazi & Mostafa Heidari Majd

  2. Student Research Committee, Zabol University of Medical Sciences, Zabol, Iran

    Amin Barani

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  1. Azam Sargazi
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  2. Amin Barani
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Correspondence to Mostafa Heidari Majd.

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Sargazi, A., Barani, A. & Heidari Majd, M. Synthesis and Apoptotic Efficacy of Biosynthesized Silver Nanoparticles Using Acacia luciana Flower Extract in MCF-7 Breast Cancer Cells: Activation of Bak1 and Bclx for Cancer Therapy. BioNanoSci. 10, 683–689 (2020). https://doi.org/10.1007/s12668-020-00753-x

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