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Antibacterial Activity and Cytotoxicity of Silver Chloride/Silver Nanocomposite Synthesized by a Bacterium Isolated from Antarctic Soil

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Abstract

This study aims to describe the antibacterial and cytotoxic properties of a nanocomposite composed of silver chloride and silver nanoparticles (AgCl/Ag) biogenically synthesized using Arthrobacter sp., a non-pathogenic Antarctic soil bacterium. Spherical nanoparticles (hydrodynamic size of 42 nm) coated with biomolecules derived from Arthrobacter sp. were obtained. The antibacterial activity of the nanocomposite was demonstrated against Gram-positive and Gram-negative bacterial strains, including the multidrug-resistant Pseudomonas aeruginosa KPC 37. Furthermore, the cytotoxicity of AgCl/Ag nanocomposite was evaluated against the non-tumoral (Vero) and tumoral (SW-1353) cell lines. The nanocomposite was not cytotoxic to the Vero cells at concentrations below 150 μg/mL, whereas at concentrations above 25 μg/mL, a significant cytotoxic effect was observed for the SW-1353 cells. Interestingly, at 150 μg/mL, the nanocomposite demonstrated a potent antibacterial activity for all bacterial strains evaluated. To the best of our knowledge, this is the first report describing the green synthesis of AgCl/Ag nanocomposite by the bacterium Arthrobacter sp. isolated from Antarctic soil. The obtained nanocomposite may find important biomedical applications, with potent antibacterial and antitumoral effects and low toxicity to healthy cells.

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Acknowledgments

We would like to thank the proofreading services for revising the text.

Funding

We have received support from FAPESP-CONICYT (2018/08194-2, 2018/02832-7), CONICYT/FONDAP/15130015, CONICYT-REDES 180003, CNPq (404815/2018-9 and 307664/2015-5), and FONDECYT 1191089. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.

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  1. Center for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Av. dos Estados 5001,, Santo André, SP, CEP 09210-580, Brazil

    Wallace R. Rolim, Joana C. Pieretti, Mônica H. M. Nascimento, Fabio F. Ferreira & Amedea B. Seabra

  2. Nanomedicine Research Unit (NANOMED), Federal University of ABC (UFABC), Santo André, SP, Brazil

    Wallace R. Rolim, Joana C. Pieretti, Mônica H. M. Nascimento, Fabio F. Ferreira & Amedea B. Seabra

  3. Center for Engineering, Modeling and Applied Social Sciences, Federal University of ABC (UFABC), Santo André, SP, Brazil

    Claudio Lamilla

  4. Department of Chemical Engineering, Universidad de La Frontera, Temuco, Chile

    Claudio Lamilla, Gonzalo R. Tortella, Maria C. Diez & Olga Rubilar

  5. Biotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), Universidad de La Frontera, Temuco, Chile

    Gonzalo R. Tortella, Maria C. Diez, Leticia Barrientos & Olga Rubilar

  6. Laboratory of Applied Molecular Biology, Universidad de La Frontera, Avenida Alemania 0458, Temuco, Chile

    Leticia Barrientos

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Rolim, W.R., Lamilla, C., Pieretti, J.C. et al. Antibacterial Activity and Cytotoxicity of Silver Chloride/Silver Nanocomposite Synthesized by a Bacterium Isolated from Antarctic Soil. BioNanoSci. 10, 136–148 (2020). https://doi.org/10.1007/s12668-019-00693-1

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