Abstract
The expression of activation markers on lymphocytes and a subpopulation of regulatory T cells (CD4+ CD25+ Hi) were evaluated in children with chronic pyelonephritis. We have distinguished the following clinical variants of pathology: dysmetabolic, obstructive, and mixed (obstructive dysmetabolic) chronic pyelonephritis. Flow cytofluorometry was used to evaluate the population and subpopulations of T lymphocytes expressing CD3+HLA-DR+ and CD8+HLA-DR+ class II histocompatibility antigens, CD95−СD3+CD95+ activation apoptosis marker, subpopulations expressing the homing receptor CD4+CD62L+ and CD4+CD62L−, and subpopulations of CD4+CD25+Hi regulatory T cells (Treg). In dysmetabolic variants of chronic pyelonephritis, an activation profile of lymphocytes is characterized mainly by positive activation and the subpopulation of CD4+ lymphocytes, forming an increase in the content of effectors/activated cells with enhanced migration into the target organ. In the case of obstructive variants of chronic pyelonephritis, antigen-mediated activation and an increase in the pool of activated cytotoxic T lymphocytes (CD8+HLA-DR+) result in activation-induced apoptosis and cell death, leading to leukopenia and lymphopenia. Expansion of regulatory T cells (CD4+CD25+ Hi) in obstructive variants of chronic pyelonephritis can be considered a factor regulating apoptosis in the process of long-term activation of T lymphocytes.
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Acknowledgments
The work was performed as part of the Competitiveness Enhancement Program of the Kazan Federal University. Authors would like to thank Mackenzie Waltke for proofreading the manuscript.
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The study was approved by the Committee on Bioethics at the Kazan State Medical University, Ministry of Health of the Russian Federation (Minutes No. 31 dated December 2, 2016). There is no conflict of interest and financial support for research.
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Agafonova, E.V., Rizvanova, F.F., Malanicheva, T.G. et al. Activation Markers and Regulatory T cells in Children with Chronic Pyelonephritis Associated with Bacterial Uropathogens. BioNanoSci. 10, 337–343 (2020). https://doi.org/10.1007/s12668-019-00676-2
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DOI: https://doi.org/10.1007/s12668-019-00676-2