, Volume 9, Issue 3, pp 672–682 | Cite as

Thermodynamics, Kinetics, and Adsorption Properties of Biomolecules onto Carbon-Based Materials Obtained from Food Wastes

  • Özkan Demirbaş
  • Mehmet Harbi Çalımlı
  • Buse Demirkan
  • Mehmet Hakkı Alma
  • Mehmet Salih NasEmail author
  • Anish KhanEmail author
  • Abdullah M. AsiriEmail author
  • Fatih ŞenEmail author


In this research, adsorption of Candida rugosa lipase enzyme (CRLE) onto activated carbon obtained from apple bark was carried out, and the thermodynamic parameters of adsorption process were investigated. The surface structural change of lipase enzyme and activated carbon was studied. The thermodynamic functions such as enthalpy, entropy, Gibbs free energy, and activation energy were investigated in their experimental work. The thermodynamic parameters of ΔG, Ea, ΔH, and ΔS were calculated as − 75.56, 13.42, − 15.29 kJ mol−1, and 202.2 J mol−1 K−1 for CRLE adsorption, respectively. The experiment results showed that the adsorption process of CRLE on activated carbon is spontaneous and exothermic. The maximum adsorption capacity according to CRLE was 5.5 pH. The maximum adsorption capacity of active carbon was found to be 96.2 mg/g at pH 5.5, 309.5 K, and initial enzyme concentration of 5.0 × 10−3 M. The protein molecules at this point are very stable that is close to the isoelectric point of lipase enzyme. As a result, we can say that the activated carbon can be used as an effective adsorbent for the adsorption of CRLE.


Activated carbon Adsorption Lipase enzyme Thermodynamic parameters 



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Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Science and LiteratureUniversity of BalikesirBalikesirTurkey
  2. 2.Tuzluca Vocational High SchoolIgdir UniversityIgdirTurkey
  3. 3.Sen Research Group, Department of Biochemistry, Faculty of Arts and ScienceDumlupınar UniversityKütahyaTurkey
  4. 4.Department of Environmental, Faculty of EngineeringUniversity of IgdirIgdirTurkey
  5. 5.Chemistry Department, Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia
  6. 6.Center of Excellence for Advanced Materials ResearchKing Abdulaziz UniversityJeddahSaudi Arabia

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