, Volume 9, Issue 2, pp 502–509 | Cite as

Analysis of the Interaction and Proliferative Activity of Adenocarcinoma, Peripheral Blood Mononuclear and Mesenchymal Stromal Cells after Co-Cultivation In Vitro

  • Kristina V. Kitaeva
  • Tikhon S. Prudnikov
  • Marina O. Gomzikova
  • Sevindzh K. Kletukhina
  • Victoria James
  • Albert A. Rizvanov
  • Valeriya V. SolovyevaEmail author


The tumor microenvironment is a heterogeneous population of cells actively involved in the process of growth and development of a tumor. Research has demonstrated the interactions between the different populations of cells are critical for the formation of the tumor microenvironment and, if recapitulated experimentally, can be used to produce more effective models for preclinical screening of anticancer drugs. In this study, we demonstrate co-culturing HeLa adenocarcinoma cells, peripheral blood mononuclear cells, and mesenchymal stromal cells results in changes in the proliferative activity of the peripheral blood mononuclear cells and mesenchymal stromal cell populations. This data supports the further development of in vitro co-culture systems utilizing these cell types for pre-clinical screening of anticancer drugs.


Co-culture Intercellular interaction Adenocarcinoma Mononuclear blood cell Mesenchymal stromal cell Test system Drug screening 


Funding Information

This study was supported by the Russian Science Foundation grant 18-74-10044. The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kristina V. Kitaeva
    • 1
  • Tikhon S. Prudnikov
    • 1
  • Marina O. Gomzikova
    • 1
  • Sevindzh K. Kletukhina
    • 1
  • Victoria James
    • 2
  • Albert A. Rizvanov
    • 1
  • Valeriya V. Solovyeva
    • 1
    Email author
  1. 1.Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussia
  2. 2.School of Veterinary Medicine and ScienceUniversity of NottinghamNottinghamUK

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