, Volume 9, Issue 1, pp 79–86 | Cite as

Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

  • Victoria V. ShumyantsevaEmail author
  • Anna A. Makhova
  • Evgenia V. Shikh
  • Tatiana V. Bulko
  • Alexey V. Kuzikov
  • Rami A. Masamrekh
  • Tatyana Shkel
  • Sergey Usanov
  • Andrei Gilep
  • Alexander I. Archakov


The electrocatalytic activity of cytochrome P450 3A4 as the main enzyme of drug metabolism was studied. Cytochrome P450 electrodes allowed to register 10−11–10−12 mol of enzyme/electrode. The study has shown that the developed electrochemical systems based on cytochromes P450 are effective non-invasive models for the analysis of drug–drug interactions at the level of biotransformation of xenobiotics. The effect of clinically significant drug combinations on the activity of cytochrome P450 3A4 was studied. The electroanalytical response of cytochrome P450 3A4 enzyme was interpreted in terms of its impact on drug interference and assessment of heme protein activity. The results can be used for modulation of cytochrome P450 activity in case of comorbid patients with polypharmacy.


Cytochrome P450 Bioelectrochemistry Mechanism-based modulators Electroanalysis Drug interactions Complex pharmacotherapy Comorbid patients Polypharmacy Therapeutic window 



The reported study was supported by Russian Fund of Fundamental Research (RFBR), research project No. 18-04-00374.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Victoria V. Shumyantseva
    • 1
    • 2
    Email author
  • Anna A. Makhova
    • 3
  • Evgenia V. Shikh
    • 3
  • Tatiana V. Bulko
    • 1
  • Alexey V. Kuzikov
    • 1
    • 2
  • Rami A. Masamrekh
    • 1
    • 2
  • Tatyana Shkel
    • 4
  • Sergey Usanov
    • 4
  • Andrei Gilep
    • 4
  • Alexander I. Archakov
    • 1
    • 2
  1. 1.Institute of Biomedical ChemistryMoscowRussia
  2. 2.Pirogov Russian National Research Medical UniversityMoscowRussia
  3. 3.Sechenov First Moscow State Medical UniversityMoscowRussia
  4. 4.Institute of Bioorganic Chemistry NASBMinskBelarus

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