, Volume 8, Issue 1, pp 473–480 | Cite as

Intravenous Transplantation of Human Umbilical Cord Blood Mononuclear Cells Overexpressing Nerve Growth Factor Improves Spatial Memory in APP/PS1 Transgenic Mice with a Model of Alzheimer’s Disease

  • M. A. Mukhamedyarov
  • A. V. Leushina
  • A. E. Tikhonova
  • E. O. Petukhova
  • E. E. Garanina
  • R. Ben Taleb
  • M. S. Kaligin
  • Y. O. Mukhamedshina
  • A. A. Rizvanov
  • A. L. Zefirov
  • R. R. Islamov


Alzheimer’s disease is a progressive incurable neurodegenerative disease manifested by dementia and other cognitive disorders. Gene-cell therapy is one of the most promising trends in the development of treatment for Alzheimer’s disease. The study was aimed to evaluate the therapeutic potential of intravenous transplantation of human umbilical cord blood mononuclear cells (UCBMCs) transduced with adenoviral vectors overexpressing nerve growth factor (NGF) for the treatment of Alzheimer’s disease in an APP/PS1 transgenic mice model. The transplantation of NGF-expressing UCBMCs was found to improve spatial memory and decrease anxiety in APP/PS1 mice. Grafted cells and their expression of NGF were detected in the cortex and hippocampus of transgenic mice in the period up to 90 days after transplantation. Thus, gene-cell therapy based on the use of NGF-overexpressing UCBMCs is a promising approach for the development of Alzheimer’s disease treatments.


Alzheimer’s disease Nerve growth factor Stem cells Gene-cell therapy APP/PS1 transgenic mice Umbilical cord blood mononuclear cells 



The study was supported by the Scholarship of the President of the Russian Federation for young researchers and scientists (СП-255.2016.4), RFFR grant no. 17-04-02175А. Some aspects of methodology for the development of gene-cell approaches to the treatment of neurodegenerative disorders were implemented with the support by the RSF grant no. 14-15-00847-П. Kazan Federal University facilities were supported by the Russian Government Program of Competitive Growth. Albert A. Rizvanov was personally supported by the state assignment 20.5175.2017/6.7 of the Ministry of Education and Science of the Russian Federation (“Leading Scientist”).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • M. A. Mukhamedyarov
    • 1
  • A. V. Leushina
    • 1
  • A. E. Tikhonova
    • 1
    • 2
  • E. O. Petukhova
    • 1
  • E. E. Garanina
    • 2
  • R. Ben Taleb
    • 1
    • 2
  • M. S. Kaligin
    • 2
  • Y. O. Mukhamedshina
    • 1
    • 2
  • A. A. Rizvanov
    • 2
  • A. L. Zefirov
    • 1
  • R. R. Islamov
    • 1
  1. 1.Kazan State Medical UniversityKazanRussia
  2. 2.Kazan (Volga Region) Federal UniversityKazanRussia

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