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Exploring the Integration of Threaded Implants: the Chemical Deep Etching Approach

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Abstract

While various techniques for analyses of the bone/implant interface are developed, most of them do not show the osseointegration process in details. In this article, we present a new inverted approach to explore the osseointegration of the dental implants, based on the chemical deep etching of titanium implants. An approach was tested on 18 implants inserted in 6 dogs. Bone/implant blocks were taken after 1, 3, and 6 months after implantation. The titanium was chemically removed from the interface, leaving the bone tissue intact. Once metal was removed, bone tissue was analyzed macroscopically and with a scanning electron microscope, afterwards decalcified and used for histological analysis. The clear patterns of implant integration into the bone tissue were obtained after 1, 3, and 6 months after implantation. After 1 month, the bone/implant interface was still very immature. After 3 months, the bone was already quite mature and organized. After 6 months, the external bone layer on the bone/implant interface appeared in its final osseointegrated form. The presented inverted method for the osseointegration analysis offers new insight into the healing process of the bone/implant interface after implantation, as well as integrative processes occurring around implants with different surfaces and designs.

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Funding

We like to acknowledge the support of this work by the Russian Science Foundation (project No. 15-14-00046) and subsidy of the Russian Government to support the Program of competitive development of Kazan Federal University.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    Fanilya A. Hafizova, Ruslan M. Mirgazizov, Rais G. Hafizov, Airat M. Mirgazizov, Dina A. Azizova, Airat R. Kayumov & Marsel Z. Mirgazizov

  2. Kazan State Medical University, Kazan, Russia

    Dmitriy E. Tsyplakov & Irek R. Hafizov

  3. Bauman Kazan State Academy of Veterinary Medicine, Kazan, Russia

    Мikhail A. Sergeev

Authors
  1. Fanilya A. Hafizova
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  2. Ruslan M. Mirgazizov
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  3. Rais G. Hafizov
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  4. Airat M. Mirgazizov
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  5. Dmitriy E. Tsyplakov
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  6. Irek R. Hafizov
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  7. Dina A. Azizova
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  8. Мikhail A. Sergeev
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  9. Airat R. Kayumov
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  10. Marsel Z. Mirgazizov
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Correspondence to Rais G. Hafizov.

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Hafizova, F.A., Mirgazizov, R.M., Hafizov, R.G. et al. Exploring the Integration of Threaded Implants: the Chemical Deep Etching Approach. BioNanoSci. 8, 313–318 (2018). https://doi.org/10.1007/s12668-017-0468-1

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  • DOI: https://doi.org/10.1007/s12668-017-0468-1

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