Fabrication of Magnetically Responsive Agarose Microbeads Doped with Live Microbial Cells

Abstract

Here, we report a scalable and rapid method to fabricate magnetically responsive agarose microgels doped with microbial cells. Low-temperature melting agarose and food-grade sunflower oil were used to fabricate microbeads during emulsification and gel setting. Microscopic algae and fungi cells were doped into ∼100-μm-sized beads as single culture or mixed. Magnetic nanoparticles were deposited either on cell walls or on bead walls. We found that the cells encapsulated in magnetically responsive microbeads were viable and able for germination.

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References

  1. 1.

    Benita, S. (1996). Microencapsulation: methods and industrial applications. New York: Marcel Dekker.

    Google Scholar 

  2. 2.

    Kailasapathy, K. (2002). Microencapsulation of probiotic bacteria: technology and potential applications. Current Issues in Intestinal Microbiology, 3, 39–48.

    Google Scholar 

  3. 3.

    Konnova, S. A., Kahraman, M., Zamaleeva, A. I., Culha, M., Paunov, V. N., Fakhrullin, R. F. (2011). Functional artificial free-standing yeast biofilms. Colloid Surface B, 88, 656–663.

    Article  Google Scholar 

  4. 4.

    de Vos, P., Lazarjani, H. A., Poncelet, D., Faas, M. M. (2014). Polymers in cell encapsulation from an enveloped cell perspective. Advanced Drug Delivery Reviews, 67–68, 15–34.

    Article  Google Scholar 

  5. 5.

    Safarik, I., Pospiskova, K., Horska, K., Safarikova, M. (2012). Potential of magnetically responsive (nano)biocomposites. Soft Matter, 8, 5407–5413.

    Article  Google Scholar 

  6. 6.

    Zamaleeva, A. I., Sharipova, I. R., Shamagsumova, R. V., Ivanov, A. N., Evtugyn, G. A., Ishmuchametova, D. G., et al. (2011). A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes. Analytical Methods, 23, 509–513.

    Article  Google Scholar 

  7. 7.

    Orive, G. R., Hernández, M., Gascón, A. R., Igartua, M., Pedraz, J. L. (2003). Survival of different cell lines in alginate-agarose microcapsules. European Journal of Pharmaceutical Sciences, 18, 23–30.

    Article  Google Scholar 

  8. 8.

    Fakhrullin, R. F., Bikmullin, A. G., Nurgaliev, D. K. (2009). Magnetically responsive calcium carbonate microcrystals. ACS Applied Materials and Interfaces, 1(9), 1847–1851.

    Article  Google Scholar 

Download references

Acknowledgments

The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.

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Correspondence to Rawil Fakhrullin.

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Konnova, S., Fakhrullin, R. Fabrication of Magnetically Responsive Agarose Microbeads Doped with Live Microbial Cells. BioNanoSci. 7, 75–77 (2017). https://doi.org/10.1007/s12668-016-0301-2

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Keywords

  • Encapsulation
  • Magnetic functionality
  • Viability
  • Agarose