, Volume 7, Issue 1, pp 75–77 | Cite as

Fabrication of Magnetically Responsive Agarose Microbeads Doped with Live Microbial Cells

  • Svetlana Konnova
  • Rawil FakhrullinEmail author


Here, we report a scalable and rapid method to fabricate magnetically responsive agarose microgels doped with microbial cells. Low-temperature melting agarose and food-grade sunflower oil were used to fabricate microbeads during emulsification and gel setting. Microscopic algae and fungi cells were doped into ∼100-μm-sized beads as single culture or mixed. Magnetic nanoparticles were deposited either on cell walls or on bead walls. We found that the cells encapsulated in magnetically responsive microbeads were viable and able for germination.


Encapsulation Magnetic functionality Viability Agarose 



The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.


  1. 1.
    Benita, S. (1996). Microencapsulation: methods and industrial applications. New York: Marcel Dekker.Google Scholar
  2. 2.
    Kailasapathy, K. (2002). Microencapsulation of probiotic bacteria: technology and potential applications. Current Issues in Intestinal Microbiology, 3, 39–48.Google Scholar
  3. 3.
    Konnova, S. A., Kahraman, M., Zamaleeva, A. I., Culha, M., Paunov, V. N., Fakhrullin, R. F. (2011). Functional artificial free-standing yeast biofilms. Colloid Surface B, 88, 656–663.CrossRefGoogle Scholar
  4. 4.
    de Vos, P., Lazarjani, H. A., Poncelet, D., Faas, M. M. (2014). Polymers in cell encapsulation from an enveloped cell perspective. Advanced Drug Delivery Reviews, 67–68, 15–34.CrossRefGoogle Scholar
  5. 5.
    Safarik, I., Pospiskova, K., Horska, K., Safarikova, M. (2012). Potential of magnetically responsive (nano)biocomposites. Soft Matter, 8, 5407–5413.CrossRefGoogle Scholar
  6. 6.
    Zamaleeva, A. I., Sharipova, I. R., Shamagsumova, R. V., Ivanov, A. N., Evtugyn, G. A., Ishmuchametova, D. G., et al. (2011). A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes. Analytical Methods, 23, 509–513.CrossRefGoogle Scholar
  7. 7.
    Orive, G. R., Hernández, M., Gascón, A. R., Igartua, M., Pedraz, J. L. (2003). Survival of different cell lines in alginate-agarose microcapsules. European Journal of Pharmaceutical Sciences, 18, 23–30.CrossRefGoogle Scholar
  8. 8.
    Fakhrullin, R. F., Bikmullin, A. G., Nurgaliev, D. K. (2009). Magnetically responsive calcium carbonate microcrystals. ACS Applied Materials and Interfaces, 1(9), 1847–1851.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Bionanotechnology LabKazan Federal UniversityKazanRussian Federation

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