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EPR Detection of DNA Interaction with 3-Carboxy-proxyl-Labelled Recombinant Human Histone H1.3

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Abstract

Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy.

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Acknowledgments

The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities. Some of the experiments were conducted using the equipment of Interdisciplinary Center for Collective Use of Kazan Federal University supported by the Ministry of Education of Russia (ID RFMEFI59414X0003) and Pharmaceutical Research and Education Center, Kazan (Volga Region) Federal University, Kazan, Russia. A. Rodionov and M. Gafurov devote this work to Dr. Igor N. Kurkin on the occasion of his 75th anniversary.

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Authors and Affiliations

  1. Kazan Federal University, 18 Kremlyovskaya St., Kazan, 420008, Russia

    Vitaly Chasov, Alexander Rodionov, Marat Gafurov, Marat Kamalov, Timur Abdullin & Albert Rizvanov

  2. Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K.I. Skryabin, Skryabin Str. 23, Moscow, 109472, Russia

    Sergey Zaitsev

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  1. Vitaly Chasov
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  2. Alexander Rodionov
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  3. Marat Gafurov
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  4. Marat Kamalov
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  5. Timur Abdullin
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  6. Sergey Zaitsev
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  7. Albert Rizvanov
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Correspondence to Vitaly Chasov.

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Chasov, V., Rodionov, A., Gafurov, M. et al. EPR Detection of DNA Interaction with 3-Carboxy-proxyl-Labelled Recombinant Human Histone H1.3. BioNanoSci. 7, 109–111 (2017). https://doi.org/10.1007/s12668-016-0294-x

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  • DOI: https://doi.org/10.1007/s12668-016-0294-x

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