Fabrication of Magnetically Modified Chlorella pyrenoidosa Microalgae Using Poly(diallyldimethyl ammonium)-stabilised Magnetic Nanoparticles

Abstract

We report fabrication of magnetically responsive Chlorella pyrenoidosa cells using poly(diallyldimethyl ammonium chloride)-stabilised iron oxide nanoparticles. The nanoparticles were characterised using transmission electron microscopy and dark-field microscopy. The interaction of magnetic nanomaterials with C. pyrenoidosa cells was studied, and high biocompability of these nanomaterials was demonstrated.

This is a preview of subscription content, log in to check access.

Fig. 1

References

  1. 1.

    Zamaleeva, A. I., Sharipova, I. R., Shamagsumova, R. V., Ivanov, A. N., Evtugyn, G. A., Ishmuchametova, D. G., et al. (2011). A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes. Analytical Methods, 23, 509–513.

    Article  Google Scholar 

  2. 2.

    Trilling, A. K., Beekwildera, J., Zuilhof, H. (2013). Antibody orientation on biosensor surfaces: a minireview. Analyst, 138, 1619–1627.

    Article  Google Scholar 

  3. 3.

    Chouteau, C., Dzyadevych, S., Chovelon, J., Durrieu, C. (2004). Development of novel conductometric biosensors based on immobilised whole cell Chlorella vulgaris microalgae. Biosensors and Bioelectronics, 19, 1089–1096.

    Article  Google Scholar 

  4. 4.

    Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S. A., Durrieu, C. (2012). Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels. Sensors, 12(12), 16879–16891.

    Article  Google Scholar 

  5. 5.

    Fakhrullin, R. F., Shlykova, L. V., Zamaleeva, A. I., Nurgaliev, D. K., Osin, Y. N., Garcia-Alonoso, J., et al. (2010). Interfacing living unicellular algae cells with biocompatible polyelectrolyte-stabilised magnetic nanoparticles. Macromolecular Bioscience, 10(10), 1257–1264.

    Article  Google Scholar 

  6. 6.

    Pack, D. W., Hoffman, A. S., Pun, S., Stayton, P. S. (2005). Design and development of polymers for gene delivery. Nature Reviews Drug Discovery, 4, 581–593.

    Article  Google Scholar 

  7. 7.

    Zhang, B. B., Wang, L., Charles, V., Rooke, J. C., Su, B. L. (2016). Robust and biocompatible hybrid matrix with controllable permeability for microalgae encapsulation. ACS Applied Materials & Interfaces, 8(14), 8939–8946.

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the Foundation for Assistance to Small Innovative Enterprises (FASIE) in the frame of UMNIK program. The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Elvira Rozhina.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Rozhina, E., Evtugyn, V., Danilushkina, A. et al. Fabrication of Magnetically Modified Chlorella pyrenoidosa Microalgae Using Poly(diallyldimethyl ammonium)-stabilised Magnetic Nanoparticles. BioNanoSci. 6, 520–522 (2016). https://doi.org/10.1007/s12668-016-0263-4

Download citation

Keywords

  • Magnetic nanoparticles
  • Chlorella pyrenoidosa
  • TEM
  • Cell surface engineering
  • Magnetic modification