Abstract
Zinc oxide nanoparticles are presently being used in cosmetics, paints, biosensors, and medical applications. However, zinc oxide nanoparticles are broadly used in the cosmetic industry for many years; recently, they have been explored for use in cancer therapy. This study was intended to comprehend the mechanism of the differential cytotoxicity of rod and spherical shaped zinc oxide nanoparticles to cervical cancer cells HeLa and SiHa. The role of ion dissolution in the toxicity of zinc oxide nanoparticles was also investigated. Zinc oxide nanorod induced significantly higher oxidative stress, reactive oxygen species compared to spherical shaped zinc oxide nanoparticles. Additionally, rod shaped nanoparticles activated the NF-κB signaling pathway. Besides, zinc oxide nanorod also induced mitochondrial membrane damage leading to apoptosis as observed by PARP cleavage and reduction of Phospho-Bad. In conclusion, zinc oxide nanorod, after proper tailoring, may have potential to use as chemotherapeutic agents against cervical cancer.
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20 August 2019
The original article unfortunately contains minor errors in Fig. 1B.
20 August 2019
The original article unfortunately contains minor errors in Fig. 1B.
Abbreviations
- NPs:
-
Nanoparticles
- ZnO NPs:
-
Zinc oxide nanoparticles
- ROS:
-
Reactive oxygen species
- MMP:
-
Mitochondrial membrane potential
- TEM:
-
Transmission electron microscope
- FE-SEM:
-
Field-emission scanning electron microscope
- DLS:
-
Dynamic light scattering
- PDI:
-
Polydispersity index
- XRD:
-
X-ray diffraction
- MTT:
-
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
- DCFDA:
-
2,7-Dichlorofluorescein di-acetate
- MDA:
-
Malondialdehyde
- LPO:
-
Lipid peroxidation
- GSH:
-
Glutathione
- PC:
-
Protein carbonyl
- SOD:
-
Superoxide dismutase
- CAT:
-
Catalase
- LDH:
-
Lactate dehydrogenase
- JC-1 dye:
-
5,5′,6,6′-Tetrachloro-1,1′,3,3-tetraethylbenzimidazolcarbocyanine iodide
- NF-κB:
-
Nuclear factor-kappa beta
- PARP:
-
Poly ADP ribose polymerase
- ARS:
-
Alizarin Red S
- OD:
-
Optical density.
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Acknowledgments
This work was financially supported by UPE (Phase II), DST-PURSE program and State Government Fellowship scheme of Jadavpur University.
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Bhattacharya, D., Bhattacharyya, A. & Karmakar, P. Evaluation of Different Oxidative Stress Parameters and Apoptosis in Human Cervical Cancer Cells Exposed to Rod and Spherical Shaped Zinc Oxide Nanoparticles. BioNanoSci. 6, 1–14 (2016). https://doi.org/10.1007/s12668-015-0186-5
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DOI: https://doi.org/10.1007/s12668-015-0186-5