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Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens

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The present study reports biologically oriented process for green synthesis of CuO nanoparticles by using eco-friendly and non-toxic Aloe vera leaf extract. Powder X-ray diffraction and transmission electron microscope analysis revealed that synthesized CuO nanoparticles are in monoclinic phase with average particle size of 20 nm. The antibacterial activity of green synthesized CuO nanoparticles was tested against three bacterial fish pathogens “viz:” Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum, which are responsible for causing severe infectious diseases in fishes. CuO NPs exhibits enhanced antibacterial activity against all the fish pathogens even at lower concentrations, i.e. above 20 μg/mL.

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  1. Nalwa, H. S. (2000). Handbook of nanostructured materials and nanotechnology. New York: Academic.

    Google Scholar 

  2. Alivisatos, A. P. (1996). Semiconductor clusters, nanocrystals, and quantum dots. Science, 271, 933–937.

    Article  Google Scholar 

  3. Siavash, I. (2011). Green synthesis of metal nanoparticles using plants. Green Chemistry, 13, 2638–2650.

    Article  Google Scholar 

  4. Das, S. K., Khan, M. M. R., Guha, A. K., Das, A. R., Mandal, A. B. (2012). Silver-nano biohybride material: synthesis, characterization and application in water purification. Bioresource Technology, 124, 495–499.

    Article  Google Scholar 

  5. Das, S. K., Dickinson, C., Lafir, F., Brougham, D. F., Marsili, E. (2012). Synthesis, characterization and catalytic activity of gold nanoparticles biosynthesized with Rhizopus oryzae protein extract. Green Chemistry, 14, 1322–1334.

    Article  Google Scholar 

  6. Das, S. K., Khan, M. M. R., Guhab, A. K., Naskar, N. (2013). Bio-inspired fabrication of silver nanoparticles on nanostructured silica: characterization and application as a highly efficient hydrogenation catalyst. Green Chemistry, 15, 2548–2557.

    Article  Google Scholar 

  7. Rao, C. N. R. (1989). Transition metal oxides. Annual Review of Physical Chemistry, 40, 291–326.

    Article  Google Scholar 

  8. Wang, Z. L. (2004). Functional oxides nanobelts—materials, properties and potential applications in nano systems and biotechnology. Annual Review of Physical Chemistry, 55, 159–196.

    Article  Google Scholar 

  9. Rakhshani, A. E. (1986). Preparation, characteristics and photovoltaic properties of cuprous oxide—a review. Solid State Electronics, 29(1), 7–17.

    Article  Google Scholar 

  10. Premkumar, T., & Geckeler, K. E. (2006). Nanosized CuO particles via a supramolecular strategy. Small, 2(5), 616–620.

    Article  Google Scholar 

  11. Ren, G., Hu, D., Cheng, E. W., Vargas-Reus, M. A., Reip, P., Allaker, R. P. (2009). Characterization of copper oxide nano particles for antimicrobial applications. International Journal of Antimicrobial Agents, 33(6), 587–590.

    Article  Google Scholar 

  12. Zailei, Z., Hongwei, C., Yingli, W., Lianying, S., Ziyi, Z., Fabing, S. (2012). Preparation of hierarchical dandelion-like CuO microspheres with enhanced catalytic performance for dimethyldichlorosilane synthesis. Catalysis Science and Technology, 2, 1953–1960.

    Article  Google Scholar 

  13. Sangeetha, G., Rajeshwari, S., Venckatesh, R. (2012). Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: optical properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 97, 1140–1144.

    Article  Google Scholar 

  14. Maensiri, S., Laokul, P., Klinkaewnarong, J., Phokha, S., Promarak, V., Seraphin, S. (2008). Indium oxide (In2O3) nanoparticles using Aloe vera plant extract: synthesis and optical properties. Journal of Optoelectronics and Advanced Materials, 10, 161–165.

    Google Scholar 

  15. Surjushe, A., Vasani, R., Saple, D. G. (2008). Aloe vera. Indian Journal of Dermatology, 53, 163–166.

    Article  Google Scholar 

  16. Vijay Kumar, P. P. N., Pammi, S. V. N., Kollu, P., Satyanarayana, K. V. V., Shameem, U. (2014). Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity. Industrial Crops and Products, 52, 562–566.

    Article  Google Scholar 

  17. Cappuccinno, J. G., & Sherman, N. (1999). Micro—a laboratory manual (pp. 254–256). Addison: Wesley Longman Inc.

  18. Stohs, S. J., & Bagchi, D. (1995). Oxidative mechanisms in the toxicity of metal ions. Free Radical Biology and Medicine, 18(2), 321–336.

    Article  Google Scholar 

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We are thankful to the DST-PURSE Programme, Advanced Analytical Laboratory, Andhra University, for the financial assistance and for their support in carrying out the research work regarding SEM-EDX and XRD analysis. The authors are thankful to CRNTS, IIT Bombay for the TEM characterization.

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Correspondence to S. V. N. Pammi.

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Kumar, P.P.N.V., Shameem, U., Kollu, P. et al. Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens. BioNanoSci. 5, 135–139 (2015).

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